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. 2025 Jul 10;17(14):2287.
doi: 10.3390/nu17142287.

Exploratory Study on Microbiota and Immune Responses to Short-Term L. paracasei CNCM I-1518 Consumption in Healthy Adults

Fernando Rivero-Pino  1   2 , Maria José Castro  3 , Paz Redondo Del Río  4 , Eloina Gutierrez  4 , Agustín Mayo-Iscar  5 , Mercedes Nocito  6 , Alfredo Corell  1   2   3
Affiliations

Exploratory Study on Microbiota and Immune Responses to Short-Term L. paracasei CNCM I-1518 Consumption in Healthy Adults

Fernando Rivero-Pino et al. Nutrients. .

Abstract

Background/objectives: The gut microbiota and immune system are interconnected, with targeted nutritional interventions offering potential to modulate immune function. This study aimed to evaluate the short-term immunomodulatory effects of Lacticaseibacillus paracasei subspecies paracasei CNCM I-1518 (L. paracasei CNCM I-1518) in healthy adults.

Methods: A 15-day dietary intervention was conducted involving healthy adults. Nutritional status, dietary habits, and systemic immune biomarkers were assessed, alongside changes in gut microbiota composition.

Results: The results revealed significant effects on both cellular and humoral immunity. Cellular immunity was enhanced through increased circulating B lymphocytes, absolute monocyte counts, and leukocyte numbers, alongside reduced eosinophil levels, potentially mitigating allergic responses. Humoral immunity was improved by elevated serum IgG1, IgG2, and IgG4 levels, enhancing defenses against pathogenic antigens, and increased serum complement proteins C3 and C4, supporting innate immunity. Microbiota analysis showed a reduction in Clostridium and the Clostridium/Escherichia coli ratio, with a notable increase in the Lactobacillus/Clostridium ratio, highlighting the strain's ability to reshape intestinal bacterial balance.

Conclusions: A short-term intake of L. paracasei CNCM I-1518 can simultaneously modulate immune function and gut microbiota composition, supporting its potential as a targeted dietary intervention to promote immune health.

Keywords: Actimel; L. paracasei; gut microbiota; immunomodulation.

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Conflict of interest statement

Danone S.A. supplied the treatment used in this investigation. None of the authors earned any money from this work.

Figures

Figure 1
Figure 1
Correlation graphics (difference LAB supplementation—basal) of (A) % of ratio of memory cytotoxic T lymphocytes vs. % of memory helper T lymphocytes (p < 0.001); (B) ratio of Clostridium/E. coli vs. IgG3 (p < 0.01); and (C) Lactobacillus/Clostridium vs. Clostridium/E. coli (p < 0.02).
See this image and copyright information in PMC

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