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. 2025 Sep 1;33(5):289-294.
doi: 10.1097/PAI.0000000000001275. Epub 2025 Aug 1.

Distinct levels of DDIT3 Amplification in Dedifferentiated Liposarcoma Developing After DDIT3 Rearrangement in Myxoid Liposarcoma

Uiree Jo  1 , Min Jeong Song, Ji-Seon Jeong, Halim Song, Joon Seon Song
Affiliations

Distinct levels of DDIT3 Amplification in Dedifferentiated Liposarcoma Developing After DDIT3 Rearrangement in Myxoid Liposarcoma

Uiree Jo et al. Appl Immunohistochem Mol Morphol. .

Abstract

Dedifferentiated liposarcoma (DDLPS) is a high-grade tumor characterized by its diverse histomorphology and development from atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDLPS). The variable morphology of DDLPS, particularly its similarity to myxoid LPS, necessitates a reliable diagnostic tool for accurate diagnosis. While DDIT3 rearrangement is a known characteristic of myxoid LPS, its amplification in DDLPS has not been extensively studied. To investigate this, we evaluated DDIT3 amplification in 29 DDLPS cases from Asan Medical Center over a 7-year period using fluorescence in situ hybridization, with immunohistochemistry for MDM2 and CDK4 performed for diagnostic confirmation. Our findings revealed DDIT3 amplification in 89.7% (26/29) of DDLPS cases, with a mean copy number of 7.4. While no significant differences in clinical characteristics or outcomes were observed between patients with and without DDIT3 amplification, patients with <4 copies of DDIT3 amplification showed a tendency toward shorter disease-free survival. These findings demonstrate the prevalence of DDIT3 amplification in DDLPS, suggesting its potential diagnostic and therapeutic significance. Additional research is necessary to fully understand the prognostic implications of DDIT3 amplification levels in DDLPS and its potential application in targeted therapies.

Keywords: FISH; dedifferentiated liposarcoma.

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Conflict of interest statement

The authors declare no conflict of interest.

References

    1. Board TWC of TE. WHO Classification of Tumours Soft Tissue and Bone Tumours, 5th ed. Lyon: IARC Press; 2020.
    1. Benchetritt M, Hofman V, Vénissac N, et al. Dedifferentiated liposarcoma of the pleura mimicking a malignant solitary fibrous tumor and associated with dedifferentiated liposarcoma of the mediastinum: Usefulness of cytogenetic and molecular genetic analyses. Cancer Genet Cytogenet. 2007;179:150–155.
    1. Lucas DR, Shukla A, Thomas DG, et al. Dedifferentiated liposarcoma with inflammatory myofibroblastic tumor-like features. Am J Surg Pathol. 2010;34:844–851.
    1. Creytens D, Libbrecht L, Ferdinande L. Nuclear expression of STAT6 in dedifferentiated liposarcomas with a solitary fibrous tumor-like morphology. Appl Immunohistochem Mol Morphol. 2015;23:462–463.
    1. Creytens D, van Gorp J, Ferdinande L, et al. Detection of MDM2/CDK4 amplification in lipomatous soft tissue tumors from formalin-fixed, paraffin-embedded tissue. Appl Immunohistochem Mol Morphol. 2015;23:126–133.

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