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. 1985 Dec;43(3 Pt 2):643-52.
doi: 10.1016/0092-8674(85)90236-3.

Reconstitution of an endocytic fusion event in a cell-free system

J Davey, S M Hurtley, G Warren

Reconstitution of an endocytic fusion event in a cell-free system

J Davey et al. Cell. 1985 Dec.

Abstract

Using a cell-free system we have obtained fusion of vesicles from the endocytic pathway. The fusion is rapid, efficient, and requires ATP. Only vesicles derived from certain positions along the endocytic pathway are capable of fusing. Lysosomes and vesicles derived from the plasma membrane do not fuse.

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