Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Aug 13;17(32):45625-45639.
doi: 10.1021/acsami.5c14143. Epub 2025 Aug 3.

Positron Emission Tomography-Based Pharmacokinetics of mRNA-Lipid Nanoparticles: A Study Quantifying the ApoE and Macrophage Contribution

Kohta Mohri  1   2 , Takayuki Miyazaki  3 , Shota Warashina  1   2 , Maiko Takahashi  1 , Qin Ren  1 , Riho Iida  2 , Yasuhiro Wada  4 , Kazuya Maeda  5 , Yasuyoshi Watanabe  4 , Yuta Suzuki  3 , Hidefumi Mukai  1   2
Affiliations

Positron Emission Tomography-Based Pharmacokinetics of mRNA-Lipid Nanoparticles: A Study Quantifying the ApoE and Macrophage Contribution

Kohta Mohri et al. ACS Appl Mater Interfaces. .

Abstract

It is important to clarify the pharmacokinetics of mRNA-loaded lipid nanoparticles (mRNA-LNPs), which have attracted attention as new pharmaceutical modalities for vaccines and therapeutic agents against various diseases. Positron emission tomography (PET) is expected to provide data to ensure the pharmacokinetics of mRNA-LNPs early after administration in detail, especially because of its high temporal resolution. In this study, we have developed a method for the preparation of 64Cu-labeled mRNA-LNPs by an approach via hybridization of short oligonucleotides to the 3' UTR region of mRNA, and conducted PET pharmacokinetic studies in normal and functionally deficient mice after intravenous and intramuscular administration, which mainly yielded the following findings. First, the common composition of mRNA-LNPs showed a clearly separated two-step accumulation process in the liver after intravenous administration in normal mice, indicating the involvement of ApoE in the second step. Second, after intravenous administration, the time to protein corona formation, in which blood proteins bind to mRNA-LNPs, was found to be within 5-10 min. Third, liver and spleen clearance was determined using integration plot analysis, and the contribution of hepatocyte uptake via ApoE to hepatic clearance after intravenous administration was approximately twice that of Kupffer cell uptake. Fourth, a comparison with luciferase reporter protein expression suggested that the protein expression ability of hepatocytes taken up via ApoE after intravenous administration and that of antigen-presenting cells transferred to the lymph nodes after intramuscular administration was remarkably high.

Keywords: 64Cu; ApoE; integration plot analysis; lipid nanoparticles; mRNA; pharmacokinetics; positron emission tomography.

PubMed Disclaimer

References

    1. Barbier A. J., Jiang A. Y., Zhang P., Wooster R., Anderson D. G.. The clinical progress of mRNA vaccines and immunotherapies. Nat. Biotechnol. 2022;40(6):840–854. doi: 10.1038/s41587-022-01294-2. - DOI - PubMed
    1. Rohner E., Yang R., Foo K. S., Goedel A., Chien K. R.. Unlocking the promise of mRNA therapeutics. Nat. Biotechnol. 2022;40(11):1586–1600. doi: 10.1038/s41587-022-01491-z. - DOI - PubMed
    1. Schoenmaker L., Witzigmann D., Kulkarni J. A., Verbeke R., Kersten G., Jiskoot W., Crommelin D. J.. mRNA-lipid nanoparticle COVID-19 vaccines: Structure and stability. Int. J. Pharm. 2021;601:120586. doi: 10.1016/j.ijpharm.2021.120586. - DOI - PMC - PubMed
    1. Domazet-Lošo T.. mRNA Vaccines: Why Is the Biology of Retroposition Ignored? Genes. 2022;13(5):719. doi: 10.3390/genes13050719. - DOI - PMC - PubMed
    1. Dagan N., Barda N., Kepten E., Miron O., Perchik S., Katz M. A., Hernán M. A., Lipsitch M., Reis B., Balicer R. D.. BNT162b2 mRNA Covid-19 Vaccine in a Nationwide Mass Vaccination Setting. N. Engl. J. Med. 2021;384(15):1412–1423. doi: 10.1056/NEJMoa2101765. - DOI - PMC - PubMed

LinkOut - more resources