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. 2025 Aug;24(8):e70343.
doi: 10.1111/jocd.70343.

Potential Cosmetic Applications of the Combined Extract of Panax ginseng, Ganoderma lucidum, Cordyceps militaris, and Several Asian Plants

Wenlong Wang  1 , Ming Zhong  1 , Ronghua Liu  2 , Shichun Zhao  1 , Xianwen Wei  1 , Qiwan Zheng  1 , Qingyu Zhong  1 , Yujie Shen  1 , Feng He  1
Affiliations

Potential Cosmetic Applications of the Combined Extract of Panax ginseng, Ganoderma lucidum, Cordyceps militaris, and Several Asian Plants

Wenlong Wang et al. J Cosmet Dermatol. 2025 Aug.

Abstract

Objective: Although bioactive compounds from single herbs are extensively explored in cosmetics, the synergistic potential of herbal combinations remains understudied. This study aimed to evaluate the stability of a combined extract of Panax ginseng, Ganoderma lucidum, Cordyceps militaris, and several Asian plants (PGC), and its multifunctional efficacy for acne-related skin dysfunction.

Methods: PGC was analyzed using high-performance liquid chromatography (HPLC) for batch consistency and bioactive quantification. Network pharmacology and molecular docking were used to identify active components and targets and assess binding affinities, respectively. In vitro assays were conducted to evaluate antibacterial activity, skin barrier repair, keratinocyte migration, reactive oxygen species (ROS) reduction, anti-inflammatory effects, and inhibition of lipid accumulation. The safety was tested via cytotoxicity assessments.

Results: HPLC analysis validated batch consistency and identified key bioactive constituents in PGC, including phenolic acids, flavonoids, and alkaloids. Integrated network pharmacology and molecular docking revealed multitarget mechanisms through the regulation of the IL-17/TNF/NF-κB axis pathway modulation. PGC exhibited potent antibacterial efficacy against acne-associated pathogens (Cutibacterium acne, MIC = 25 μg/mL), restored skin barrier integrity (filaggrin, +235%; loricrin, +261%), and sodium dodecyl sulfate (SDS)-induced damage (85%). Concurrently, PGC accelerated keratinocyte migration (40%), reduced ROS (45%) and abnormal lipid droplet content (60%), and attenuated inflammatory responses (40% nitric oxide (NO) inhibition) while maintaining biosafety (no cytotoxicity ≤ 200 μg/mL).

Conclusion: PGC exemplified the translational potential of herbal compatibility, offering a multitargets solution for acne management through integrating antibacterial, barrier-repair, anti-inflammatory actions, and several other effects. This study established a network pharmacology-guided framework for developing evidence-based multitargets cosmetics.

Keywords: cell culture; computer modeling; cosmetic formulation; herbal synergy; multitargets efficacy; skin barrier.

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Conflict of interest statement

All authors, except Ronghua Liu, were employed by Lasur Cosmetics Co. Ltd. The authors declare that the research was conducted without any commercial or financial relationships that could be perceived as potential conflicts of interest. They agree to submit the manuscript to “Journal of Cosmetic Dermatology” as an original research article. This manuscript has not been published elsewhere and is not currently under consideration by any other journals.

Figures

FIGURE 1
FIGURE 1
Research design.
FIGURE 2
FIGURE 2
The fingerprints for the 10 PGC batches.
FIGURE 3
FIGURE 3
Venn diagram of PGC targets and application associated targets.
FIGURE 4
FIGURE 4
The “PGC‐Components‐Targets‐Applications” network diagram. Round rectangles denote the active compounds, whereas ellipse nodes indicate targets. The size of nodes is associated with their respective degree value.
FIGURE 5
FIGURE 5
The PPI network of hub targets.
FIGURE 6
FIGURE 6
Bubble charts of (a) GO term enrichment analysis and (b) KEGG pathway enrichment analysis.
FIGURE 7
FIGURE 7
Molecular docking visualization (a) Potential binding position between bicuculline and HSP90AA1. (b) Potential binding position between frutinone A and HSP90AA1. (c) Potential binding position between fumarine and HSP90AA1.
FIGURE 8
FIGURE 8
The effect of varying PGC concentrations on cellular activity. (a) HaCaT. (b) RAW 264.7. (c) SZ95.
FIGURE 9
FIGURE 9
Effect of PGC on cell activity after SDS stimulation. Each bar represents the mean ± SD of study (n = 6); versus BC, p** < 0.01; versus NC, p ## < 0.01 when compared between two groups (Student's t‐test).
FIGURE 10
FIGURE 10
Effects of PGC on FLG and LOR expression. (a) Integrated optical density of LOR. (b) Integrated optical density of FLG. Each bar represents the mean ± SD of study (n = 6); versus BC, p** < 0.01; versus NC, p ## < 0.01 when compared between two groups (Student's t‐test).
FIGURE 11
FIGURE 11
Effect of PGC on migration rate. Each bar represents the mean ± SD of study (n = 6), versus 1% FBS, p** < 0.01; versus 10% FBS, 0.01 < p # < 0.05, p ## < 0.01 when compared between two groups (Student's t‐test).
FIGURE 12
FIGURE 12
Oil control properties of PGC after DHT and PA induction. Each bar represents the mean ± SD of study (n = 6), versus BC, p** < 0.01; versus NC, 0.01 < p # < 0.05, p ## < 0.01 when compared between two groups (Student's t‐test).
FIGURE 13
FIGURE 13
Variations in NO levels within the supernatant of cell cultures. Each bar represents the mean ± SD of study (n = 6), versus BC, p** < 0.01; versus NC, p ## < 0.01 when compared between two groups (Student's t‐test).
FIGURE 14
FIGURE 14
The antioxidant properties of PGCs after H2O2 stimulation. Each bar represents the mean ± SD of study (n = 6), versus BC, p** < 0.01; versus NC, p ## < 0.01 when compared between two groups (Student's t‐test).
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References

    1. Hay R. J., Johns N. E., Williams H. C., et al., “The Global Burden of Skin Disease in 2010: An Analysis of the Prevalence and Impact of Skin Conditions,” Journal of Investigative Dermatology 134 (2014): 1527–1534. - PubMed
    1. Franik G., Bizoń A., Włoch S., Kowalczyk K., Biernacka‐Bartnik A., and Madej P., “Hormonal and Metabolic Aspects of Acne Vulgaris in Women With Polycystic Ovary Syndrome,” European Review for Medical and Pharmacological Sciences 22 (2018): 4411. - PubMed
    1. Harris V. R. and Cooper A. J., “Modern Management of Acne,” Medical Journal of Australia 206 (2017): 41–45. - PubMed
    1. Tsai H., Lee W., Wang P., Cheng K., Chen Y., and Shen S., “ Propionibacterium acnes‐Induced iNOS and COX‐2 Protein Expression via ROS‐Dependent NF‐κB and AP‐1 Activation in Macrophages,” Journal of Dermatological Science 69 (2013): 122–131. - PubMed
    1. Vijayanand P., Seumois G., Simpson L. J., et al., “Interleukin‐4 Production by Follicular Helper T Cells Requires the Conserved Il4 Enhancer Hypersensitivity Site V,” Immunity 36 (2012): 175–187. - PMC - PubMed