Fixation of platelets for scanning and transmission electron microscopy
- PMID: 407672
- DOI: 10.3109/10520297709116767
Fixation of platelets for scanning and transmission electron microscopy
Abstract
A double fixation method of preparing platelet suspensions for both scanning and transmission electron microscopy is outlined. Prefixation in 0.1% glutaraldehyde allows for immediate preservation of morphologic characteristics induced by experimental procedures, but does not completely destroy platelet surface stickiness. Preservation of surface stickiness allows subsequent production of a platelet pellet for processing for transmission electron microscopy. This pelleting cannot be achieved when higher initial concentrations of glutaraldehyde are used for prefixation. Prefixation in 0.1% glutaraldehyde is also an appropriate initial step for preservation of platelets in suspension for scanning electron microscopy.
Similar articles
-
A reliable method with good cell preservation for the demonstration of peroxidase activity in human platelets and megakaryocytes.Histochemistry. 1984;80(1):79-84. doi: 10.1007/BF00492775. Histochemistry. 1984. PMID: 6199333
-
The substructure of marginal bundles in human blood platelets.J Ultrastruct Mol Struct Res. 1988 Jun;99(3):244-53. doi: 10.1016/0889-1605(88)90068-7. J Ultrastruct Mol Struct Res. 1988. PMID: 3143769
-
Roles of fixatives and cryoprotectants in cryotechniques for electron microscopy: effects of glutaraldehyde and glycerol on structures and functions of platelets.J Electron Microsc (Tokyo). 1981;30(1):69-71. J Electron Microsc (Tokyo). 1981. PMID: 6793682 No abstract available.
-
Use of scanning electron microscopy to study structural-functional relationships in normal and diseased platelets.Scan Electron Microsc. 1985;(Pt 1):357-91. Scan Electron Microsc. 1985. PMID: 3890145 Review.
-
Preparation of cytoskeletons of cells in culture for high resolution scanning and scanning transmission electron microscopy.Scanning Microsc. 1988 Sep;2(3):1647-61. Scanning Microsc. 1988. PMID: 3059482 Review.
Cited by
-
Topography of UV-Melanized Thalli of Lobaria pulmonaria (L.) Hoffm.Plants (Basel). 2023 Jul 12;12(14):2627. doi: 10.3390/plants12142627. Plants (Basel). 2023. PMID: 37514242 Free PMC article.
-
Shape change of blood platelets induced by myelin basic protein.Experientia. 1979 Aug 15;35(8):1081-3. doi: 10.1007/BF01949954. Experientia. 1979. PMID: 89969
-
Fixation and embedding of small volumes of platelets for transmission electron microscopy.J Clin Pathol. 1980 Jun;33(6):600-2. doi: 10.1136/jcp.33.6.600. J Clin Pathol. 1980. PMID: 6995499 Free PMC article. No abstract available.
-
Shape change reaction of platelets in protein-free medium: ultramorphology.Experientia. 1979 Jun 15;35(6):840-2. doi: 10.1007/BF01968285. Experientia. 1979. PMID: 467611