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. 1977 May;52(3):151-8.
doi: 10.3109/10520297709116767.

Fixation of platelets for scanning and transmission electron microscopy

Fixation of platelets for scanning and transmission electron microscopy

J C Mattson et al. Stain Technol. 1977 May.

Abstract

A double fixation method of preparing platelet suspensions for both scanning and transmission electron microscopy is outlined. Prefixation in 0.1% glutaraldehyde allows for immediate preservation of morphologic characteristics induced by experimental procedures, but does not completely destroy platelet surface stickiness. Preservation of surface stickiness allows subsequent production of a platelet pellet for processing for transmission electron microscopy. This pelleting cannot be achieved when higher initial concentrations of glutaraldehyde are used for prefixation. Prefixation in 0.1% glutaraldehyde is also an appropriate initial step for preservation of platelets in suspension for scanning electron microscopy.

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