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. 2025 Jul 23:12:1610063.
doi: 10.3389/fmed.2025.1610063. eCollection 2025.

Global trends in the application of nanopore sequencing technology in the detection of infectious disease pathogens: a bibliometric analysis from 2014 to 2024

Jiali Long  1   2 , Benhua Zeng  1   2 , Jia Li  1   2 , Juan Zhang  1   2 , Guohong Deng  1   2
Affiliations

Global trends in the application of nanopore sequencing technology in the detection of infectious disease pathogens: a bibliometric analysis from 2014 to 2024

Jiali Long et al. Front Med (Lausanne). .

Abstract

Purpose: This study aimed to comprehensively analyze the global landscape, trends, and research focus of nanopore sequencing technology in the field of pathogenic microorganism diagnosis using bibliometric analysis.

Methods: Literature published between January 2014, and December 2024, was retrieved from the Web of Science Core Collection. A cross-sectional bibliometric analysis was conducted using VOSviewer, CiteSpace, Origin 2024, and R software to extract and evaluate metrics. Publications were categorized by country, institution, author, journal, highly cited papers, and keywords. Variables were compared based on publication output and academic impact, which included citation counts, citation impact, H-index, journal impact factor, total link strength, major pathogens, and research directions.

Results: Initial searches identified 2,098 articles related to nanopore sequencing and pathogenic microorganisms, of which 729 were ultimately included in the analysis. Among the 104 participating countries, the United Kingdom, the United States, and China have led in publication output, citations, and academic influence. The most versatile institution was the University of Oxford, followed by Zhejiang University. The most productive scholars and journals were Crook, Derrick W., and Frontiers in Microbiology, respectively. Keyword analysis revealed that the primary advantages of nanopore sequencing include portability, long-read capabilities, and real-time analysis. Current research hotspots focus on real-time pathogen identification, viral genomic surveillance, and antimicrobial resistance profiling.

Conclusion: Presently, nanopore sequencing is rapidly transitioning from laboratory research to on-site sequencing and public health emergency scenarios. To our knowledge, this study is the first bibliometric analysis to comprehensively delineate the latest developments in nanopore sequencing in pathogenic microorganism diagnosis. It provides researchers with an understanding of the current situation, identifies knowledge gaps, and points out future research directions.

Keywords: antimicrobial resistance; bibliometric analysis; genomic surveillance; nanopore sequencing; pathogenic microorganisms; real-time.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Flowchart of metrology screening.
Figure 2
Figure 2
Baseline bibliometric characteristics of all eligible documents. (A) Annual publications and citations. (B) Citation impact per year and H-index. (C) Research areas.
Figure 3
Figure 3
Relationships and clusters of countries. (A) Classification of countries according to income levels. (B) Geographical distribution based on the number of documents. (C) Relationship between international and domestic collaborations.
Figure 4
Figure 4
Visualization of institutions. (A) Collaboration network of institutions. (B) Top 10 institutions for related publications.
Figure 5
Figure 5
Visualization of authors and journals (A,B).
Figure 6
Figure 6
Keyword analysis. (A) Cluster visualization map of keywords analysis. (B) Top 25 keywords with the strongest citation bursts generated. (C) Keyword timeline clustering map.
See this image and copyright information in PMC

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