Comparative analysis of MAIPA assay and PakAuto assay for the diagnosis of primary immune thrombocytopenia
- PMID: 40775095
- PMCID: PMC12331841
- DOI: 10.1007/s10238-025-01831-8
Comparative analysis of MAIPA assay and PakAuto assay for the diagnosis of primary immune thrombocytopenia
Abstract
The detection of platelet-specific antibodies plays an important role in the clinical diagnosis of primary immune thrombocytopenia (ITP). MAIPA assay and PakAuto assay are the two main methods for this purpose. However, a comprehensive comparative evaluation of these techniques in clinical practice remains lacking. In this study, we collected 48 patients with thrombocytopenia. MAIPA and PakAuto are used to examine platelet-specific antibodies synchronously. Based on methodologies and analysis of experimental results, we conclude that PakAuto assay with a high sensitivity and accuracy can offer higher throughput and broader human platelet antibodies detection capabilities, making it more suitable for screening.
Keywords: Monoclonal antibody immobilization of platelet antigens assay (MAIPA); Platelet glycoprotein-specific assay (PakAuto); Primary immune thrombocytopenia (ITP).
© 2025. The Author(s).
Conflict of interest statement
Declarations. Conflict of interest: The authors have no relevant financial or non-financial interests to disclose. Informed consent: Informed consent was obtained from all individual participants included in this study, or their legal guardians or wards.
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References
-
- Moulis G, Palmaro A, Montastruc JL, et al. Epidemiology of incident immune thrombocytopenia: a nationwide population-based study in France. Blood. 2014;124(22):3308–15. - PubMed
-
- Lee JY, Lee JH, Lee H, et al. Epidemiology and management of primary immune thrombocytopenia: a nationwide population-based study in Korea. Thromb Res. 2017;155:86–91. - PubMed
-
- Kuwana M, Kurata Y, Fujimura K, et al. Preliminary laboratory based diagnostic criteria for immune thrombocytopenic purpura: evaluation by multi-center prospective study. J Thromb Haemost. 2006;4(9):1936–43. - PubMed
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