Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2025 Aug 7;25(1):282.
doi: 10.1007/s10238-025-01831-8.

Comparative analysis of MAIPA assay and PakAuto assay for the diagnosis of primary immune thrombocytopenia

Affiliations
Comparative Study

Comparative analysis of MAIPA assay and PakAuto assay for the diagnosis of primary immune thrombocytopenia

Lixuan Chen et al. Clin Exp Med. .

Abstract

The detection of platelet-specific antibodies plays an important role in the clinical diagnosis of primary immune thrombocytopenia (ITP). MAIPA assay and PakAuto assay are the two main methods for this purpose. However, a comprehensive comparative evaluation of these techniques in clinical practice remains lacking. In this study, we collected 48 patients with thrombocytopenia. MAIPA and PakAuto are used to examine platelet-specific antibodies synchronously. Based on methodologies and analysis of experimental results, we conclude that PakAuto assay with a high sensitivity and accuracy can offer higher throughput and broader human platelet antibodies detection capabilities, making it more suitable for screening.

Keywords: Monoclonal antibody immobilization of platelet antigens assay (MAIPA); Platelet glycoprotein-specific assay (PakAuto); Primary immune thrombocytopenia (ITP).

PubMed Disclaimer

Conflict of interest statement

Declarations. Conflict of interest: The authors have no relevant financial or non-financial interests to disclose. Informed consent: Informed consent was obtained from all individual participants included in this study, or their legal guardians or wards.

Figures

Fig. 1
Fig. 1
Diagnostic classifications of study patients (A. Classification in 31 ITP patients. B. Platelet count in patients.)
Fig. 2
Fig. 2
Patients with positive platelet-specific antibodies in the MAIPA assay and PakAuto assay

Similar articles

References

    1. Moulis G, Palmaro A, Montastruc JL, et al. Epidemiology of incident immune thrombocytopenia: a nationwide population-based study in France. Blood. 2014;124(22):3308–15. - PubMed
    1. Lee JY, Lee JH, Lee H, et al. Epidemiology and management of primary immune thrombocytopenia: a nationwide population-based study in Korea. Thromb Res. 2017;155:86–91. - PubMed
    1. Kuwana M, Kurata Y, Fujimura K, et al. Preliminary laboratory based diagnostic criteria for immune thrombocytopenic purpura: evaluation by multi-center prospective study. J Thromb Haemost. 2006;4(9):1936–43. - PubMed
    1. Arnold DM, Nazy I, Clare R, et al. Misdiagnosis of primary immune thrombocytopenia and frequency of bleeding: lessons from the McMaster ITP Registry. Blood Adv. 2017;1(25):2414–20. - PMC - PubMed
    1. Miltiadous O, Hou M, Bussel JB. Identifying and treating refractory ITP: difficulty in diagnosis and role of combination treatment. Blood. 2020;135(7):472–90. - PMC - PubMed

LinkOut - more resources