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Comparative Study
. 2025 Aug 7;25(1):282.
doi: 10.1007/s10238-025-01831-8.

Comparative analysis of MAIPA assay and PakAuto assay for the diagnosis of primary immune thrombocytopenia

Lixuan Chen #  1 , Chaolin Chen #  2 , Rui Tuo  3   4 , Limei Ye  2 , Shuting Ouyang  2 , Hao Su  2 , Jianxi Lai  2 , Ying Feng  5 , Yang Xiao  6
Affiliations
Comparative Study

Comparative analysis of MAIPA assay and PakAuto assay for the diagnosis of primary immune thrombocytopenia

Lixuan Chen et al. Clin Exp Med. .

Abstract

The detection of platelet-specific antibodies plays an important role in the clinical diagnosis of primary immune thrombocytopenia (ITP). MAIPA assay and PakAuto assay are the two main methods for this purpose. However, a comprehensive comparative evaluation of these techniques in clinical practice remains lacking. In this study, we collected 48 patients with thrombocytopenia. MAIPA and PakAuto are used to examine platelet-specific antibodies synchronously. Based on methodologies and analysis of experimental results, we conclude that PakAuto assay with a high sensitivity and accuracy can offer higher throughput and broader human platelet antibodies detection capabilities, making it more suitable for screening.

Keywords: Monoclonal antibody immobilization of platelet antigens assay (MAIPA); Platelet glycoprotein-specific assay (PakAuto); Primary immune thrombocytopenia (ITP).

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Conflict of interest statement

Declarations. Conflict of interest: The authors have no relevant financial or non-financial interests to disclose. Informed consent: Informed consent was obtained from all individual participants included in this study, or their legal guardians or wards.

Figures

Fig. 1
Fig. 1
Diagnostic classifications of study patients (A. Classification in 31 ITP patients. B. Platelet count in patients.)
Fig. 2
Fig. 2
Patients with positive platelet-specific antibodies in the MAIPA assay and PakAuto assay
See this image and copyright information in PMC

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