Determination of phenylethanolamine N-methyltransferase by high-performance liquid chromatography with fluorescence detection
- PMID: 4077946
- DOI: 10.1016/s0021-9673(01)92479-3
Determination of phenylethanolamine N-methyltransferase by high-performance liquid chromatography with fluorescence detection
Abstract
A highly sensitive assay method for phenylethanolamine N-methyltransferase in rat adrenal medulla and brain is described which employs high-performance liquid chromatography with fluorescence detection. Epinephrine formed enzymatically from the substrate norepinephrine and isoproterenol (internal standard), after chromatography on a small cartridge of a cation exchanger, Toyopak SP, are converted into the corresponding fluorescent compounds by reaction with 1,2-diphenylethylenediamine, a selective fluorescence derivatization reagent for catechol compounds. The derivatives are separated by reversed-phase chromatography on TSK gel ODS-120T. The detection limit for epinephrine formed enzymatically is 0.66 pmol per assay tube.
Similar articles
-
Assay for tyrosine hydroxylase by high-performance liquid chromatography with fluorescence detection.J Chromatogr. 1987 Apr 10;415(2):289-96. doi: 10.1016/s0378-4347(00)83220-x. J Chromatogr. 1987. PMID: 2884228
-
High-performance liquid chromatography of plasma catecholamines using 1,2-diphenylethylenediamine as precolumn fluorescence derivatization reagent.J Chromatogr. 1985 Nov 8;344:61-70. doi: 10.1016/s0378-4347(00)82007-1. J Chromatogr. 1985. PMID: 3003137
-
Assay for dopamine beta-hydroxylase in human plasma and rat serum by high-performance liquid chromatography with fluorimetric detection.J Chromatogr. 1987 Oct 30;421(2):237-44. doi: 10.1016/0378-4347(87)80404-8. J Chromatogr. 1987. PMID: 3429580
-
Adrenocortical control of the biosynthesis of epinephrine and proteins in the adrenal medulla.Pharmacol Rev. 1972 Jun;24(2):411-26. Pharmacol Rev. 1972. PMID: 4117970 Review. No abstract available.
-
[Analyses of biogenic related compounds based on intramolecular excimer-forming fluorescence derivatization].Yakugaku Zasshi. 2003 Aug;123(8):691-6. doi: 10.1248/yakushi.123.691. Yakugaku Zasshi. 2003. PMID: 12931665 Review. Japanese.
Cited by
-
Double stable isotope ultra performance liquid chromatographic-tandem mass spectrometric quantification of tissue content and activity of phenylethanolamine N-methyltransferase, the crucial enzyme responsible for synthesis of epinephrine.Anal Bioanal Chem. 2013 Feb;405(5):1713-9. doi: 10.1007/s00216-012-6599-x. Epub 2012 Dec 8. Anal Bioanal Chem. 2013. PMID: 23224622 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Research Materials