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. 2025 Jul 22;87(8):4848-4852.
doi: 10.1097/MS9.0000000000003572. eCollection 2025 Aug.

Effect of Sheng Xuexiaoban capsules on Tregs in an ITP mouse model

Jianqiang Liu  1 , Xiaoyan Li  2 , Shenggui Hou  3 , Aixia Dou  2
Affiliations

Effect of Sheng Xuexiaoban capsules on Tregs in an ITP mouse model

Jianqiang Liu et al. Ann Med Surg (Lond). .

Abstract

Objectives: To study the effect of Sheng Xuexiaoban capsules (SXXCs) on restoring immune dysfunction in ITP model mice.

Methods: We assessed changes in platelet counts, megakaryocyte counts, Treg functions, interleukin-2 expression, and other indicators to investigate the effect of SXXCs in the ITP mouse model.

Results: After treatment of the ITP mouse model with SXXCs, we found increases in platelets (577.35 ± 76.94 vs. 123.33 ± 16.24, P < 0.05) and the Treg number (7.35 ± 1.94 vs. 1.33 ± 1.24), and Foxp3 mRNA expression (2-ΔCt: 0.2493 ± 0.03102 vs. 0.683 ± 0.05774, P = 0.0027). Decreased interleukin-2 produced by effector T cells was detected (2-ΔCt: 0.6119 ± 0.01173 vs. 2.838 ± 0.1004, P < 0.001). SXXCs treatment significantly improved the proportion and function of Tregs. By increasing Foxp3 mRNA expression and decreasing interleukin-2, we restored immune tolerance to achieve a good therapeutic effect in ITP model mice.

Conclusions: We confirmed that SXXCs have a good therapeutic effect on ITP. Simultaneously we verified the important role of Tregs in ITP pathogenesis.

Keywords: ITP mouse model; Tregs; immune thrombocytopenia.

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Conflict of interest statement

Sponsorships or competing interests that may be relevant to content are disclosed at the end of this article. The authors declare that they have no conflicts of interest to report regarding the present study.

Figures

Figure 1.
Figure 1.
Real-time PCR of Foxp3 mRNA in isolated Tregs of each group. Compared with the normal group, Foxp3 mRNA expression in ITP mice was decreased significantly (P < 0.0027). Compared with the ITP model group, Foxp3 mRNA expression was significantly upregulated in the SSXCs group (2ΔCt: 0.2493 ± 0.03102 vs. 0.683 ± 0.05774, P = 0.0027).
Figure 2.
Figure 2.
Real-time PCR of IL-2 mRNA in isolated Teff cells of each group. Compared with the normal group, IL-2 mRNA expression in the ITP group was increased significantly (P < 0.0001). IL-2 mRNA expression was significantly downregulated in the SSXCs group (2ΔCt: 0.6119 ± 0.01173 vs. 2.838 ± 0.1004, P < 0.001), and Prednisone group (P < 0.0001) compared with the ITP group.
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