Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Aug 6;15(15):2300.
doi: 10.3390/ani15152300.

Genetic Effects of Chicken Pre-miR-3528 SNP on Growth Performance, Meat Quality Traits, and Serum Enzyme Activities

Jianzhou Shi  1   2   3   4 , Jinbing Zhao  1 , Bingxue Dong  1 , Na Li  1 , Lunguang Yao  1 , Guirong Sun  2   5
Affiliations

Genetic Effects of Chicken Pre-miR-3528 SNP on Growth Performance, Meat Quality Traits, and Serum Enzyme Activities

Jianzhou Shi et al. Animals (Basel). .

Abstract

The aim was to investigate the genetic effects of a SNP located in the precursor region of gga-miR-3528. (1) Single-nucleotide polymorphisms within precursor regions of microRNAs play crucial biological roles. (2) Utilizing a Gushi-Anka F2 resource population (n = 860), we screened and validated miRNA SNPs. A SNP mutation in the miR-3528 precursor region was identified. Specific primers were designed to amplify the polymorphic fragment. Genotyping was performed for this individual SNP across the population, using the MassArray system. Association analyses were conducted between this SNP and chicken growth and body measurement traits, carcass traits, meat quality traits, and serum enzyme activities. (3) The rs14098602 (+12 bp A > G) was identified within the precursor region of gga-miR-3528. Significant associations (p < 0.05) were observed between this SNP and chicken growth traits (body weight at the age of 0 day, body weight at the age of 2 weeks, and body weight at the age of 4 weeks), carcass traits (evisceration weight), meat quality traits (subcutaneous fat rate and pectoral muscle density), and serum enzyme activities (total protein, albumin, globulin, cholinesterase, and lactate dehydrogenase). (4) These findings suggest that the polymorphism at rs14098602 may influence chicken growth, meat quality, and serum biochemical indices, through specific mechanisms. The gga-miR-3528 gene likely plays an important role in chicken development. Therefore, this SNP can serve as a molecular marker for genetic breeding and auxiliary selection of growth-related traits, facilitating the rapid establishment of elite chicken populations with superior genetic resources.

Keywords: F2 resource population; carcass traits; growth performance; meat quality traits; microRNAs (miRNAs); pre-miRNA-3528; serum enzyme activities; single-nucleotide polymorphism (SNP).

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure 1
Figure 1
PCR products. M: marker; 1–10: lanes.
Figure 2
Figure 2
The partial DNA sequencing results of miR-3528 precursor region. Arrow: SNP rs14098602 (A > G) (+88 bp). The area pointed to by the arrow represents the mutation site.
Figure 3
Figure 3
Sequence result of PCR products. The shaded parts represent primers, the yellow underlined parts represent miRNA-3528 sequences, and the boxes represent mutants.
Figure 4
Figure 4
Characteristic mass spectra of different genotypes of gga-miR-3258. Structural prediction of pre-miR-3528. (a) AA genotype; (b) GG genotype; (c) AG genotype.
Figure 4
Figure 4
Characteristic mass spectra of different genotypes of gga-miR-3258. Structural prediction of pre-miR-3528. (a) AA genotype; (b) GG genotype; (c) AG genotype.
Figure 5
Figure 5
Prediction of the secondary structure of different alleles (G/A) of miRNA-3528. (a) Wild type; (b) mutant type. The area marked by the red circle represents the site of genetic mutation.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Wang Y., Ru J., Meng X., Song J., Jiang Q., Li S., Jiang J., Li Y., Ali H.H. Role of SNPs in the Biogenesis of Mature miRNAs. BioMed Res. Int. 2021;2021:2403418. doi: 10.1155/2021/2403418. - DOI - PMC - PubMed
    1. Bhatia A., Upadhyay A.K., Sharma S. Screening and analysis of single nucleotide polymorphism in the 3′-UTR microRNA target regions and its implications for lung tumorigenesis. Pharmacogenomics. 2024;25:299–314. doi: 10.1080/14622416.2024.2355864. - DOI - PMC - PubMed
    1. Li Y., Li C., Liu S., Yang J., Shi L., Yao Y. The associations and roles of microRNA single-nucleotide polymorphisms in cervical cancer. Int. J. Med. Sci. 2021;18:2347–2354. doi: 10.7150/ijms.57990. - DOI - PMC - PubMed
    1. Rykova E., Ershov N., Damarov I., Merkulova T. SNPs in 3′UTR miRNA Target Sequences Associated with Individual Drug Susceptibility. Int. J. Mol. Sci. 2022;23:13725. doi: 10.3390/ijms232213725. - DOI - PMC - PubMed
    1. Wu K., Kong F., Zhang J., Tang Y., Chen Y., Chao L., Nie L., Huang Z. Recent Progress in Single-Nucleotide Polymorphism Biosensors. Biosensors. 2023;13:864. doi: 10.3390/bios13090864. - DOI - PMC - PubMed

LinkOut - more resources