Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Dec 20;13(24):8955-67.
doi: 10.1093/nar/13.24.8955.

Interaction of DAPI with double-stranded ribonucleic acids

G Manzini, L Xodo, M L Barcellona, F Quadrifoglio
Free PMC article

Interaction of DAPI with double-stranded ribonucleic acids

G Manzini et al. Nucleic Acids Res. .
Free PMC article

Abstract

The interaction of DAPI with natural and synthetic double-stranded polyribonucleotides was studied with different optical and calorimetric methods. The results were similar to those obtained previously with double-stranded polydeoxynucleotides, i.e. two interaction modes, the first of which shows high affinity for AU clusters and consequent strong fluorescence enhancement. The results suggest caution in the use of DAPI as selective fluorescent staining agent for DNA in the presence of RNA. A narrow groove binding model with hydrogen bonds between DAPI and AU pairs is proposed. An intercalation mechanism can be excluded because of the non planarity of DAPI molecule.

PubMed Disclaimer

References

    1. J Mol Biol. 1974 Aug 25;87(4):817-33 - PubMed
    1. J Biomol Struct Dyn. 1984 Jun;1(6):1457-72 - PubMed
    1. Exp Cell Res. 1976 Mar 15;98(2):411-23 - PubMed
    1. Exp Cell Res. 1978 Feb;111(2):327-32 - PubMed
    1. Nucleic Acids Res. 1978 Oct;5(10):3775-99 - PubMed

Publication types