Correction: Yun et al. Rhusflavone Modulates Osteoclastogenesis Through RANKL-Induced AKT Signaling in Bone Marrow-Derived Macrophages. Int. J. Mol. Sci. 2025, 26, 3025

Abstract

In the original publication [...].

Figure 5

Effects of Rhus on the inhibition of receptor activator of nuclear factor kappa B ligand (RANKL)-induced AKT signaling pathway in osteoclastogenesis. (A) Bone marrow macrophages (BMMs) were differentiated with 10 μM Rhus, in the presence or absence of 1 μM SC79 or LY294002, for 1 day. Western blot analysis was used to assess c-Fos and β-actin expression levels. The relative level (%) is presented as a bar graph. (B,C) The BMMs were cultured in 30 ng/mL M-CSF and 100 ng/mL RANKL with 10 μM Rhus, in the presence or absence of 1 μM SC79 or LY294002, for 5 days. Mature osteoclasts were detected with tartrate-resistant acid phosphatase (TRAP) staining (pink) (B). TRAP-positive multinucleated osteoclasts (MNCs) were counted under a microscope (C). A quantity of 3–10 nuclei (left), 10 < nuclei (right). Scale bar: 100 μm. *, p < 0.05 compared with RANKL alone. #, p < 0.05 compared with RANKL + Rhus. The data presented are derived from three separate experiments and are expressed as the mean ± standard deviation (SD).