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. 2025 Jul 26;30(15):3142.
doi: 10.3390/molecules30153142.

Anti- Helicobacter pylori and Anti-Inflammatory Sesquiterpenoids from the Rhizoma of Atractylodes macrocephala

So Yeong Jeong  1 , Dong-Min Kang  2 , Hyun-Jun Kim  3 , Sang Won Yeon  1 , Hak Hyun Lee  1 , Min Hee Kim  1 , Bang Yeon Hwang  1 , Mi-Jeong Ahn  2 , Mi Kyeong Lee  1
Affiliations

Anti- Helicobacter pylori and Anti-Inflammatory Sesquiterpenoids from the Rhizoma of Atractylodes macrocephala

So Yeong Jeong et al. Molecules. .

Abstract

Helicobacter pylori, a spiral-shaped bacterium found in the stomach, is associated with various gastrointestinal and systemic health conditions. Effective suppression of H. pylori is therefore critical for managing gastrointestinal diseases. In a search for natural products with anti-H. pylori activity, the extract of Atractylodes macrocephala rhizoma showed significant inhibitory effects. Chromatographic purification of A. macrocephala extract yielded thirteen compounds, which were identified as ten sesquiterpenes and three polyacetylenes by spectroscopic analysis. The sesquiterpene compounds belong to the eudesmane or eudesmane lactone types and exhibited structure-dependent efficacy. The major eudesmane lactone sesquiterpene, atractylenolide I (1), showed strong inhibitory activity comparable to metronidazole, a positive control, and atractylenolide III (3) also showed good efficacy. However, structural modification such as hydroxylation, methylation, or acetylation of the sesquiterpenes led to reduced activity. In contrast, polyacetylene derivatives displayed only mild inhibitory effects. Further evaluation of the active compounds against three H. pylori strains such as 51, 43504, and 26695 showed that atractylenolide I (1) had potent inhibitory effects against all three strains, with MIC50 values of ranging from 27.3 to 48.6 μM and MIC90 values from 45.4 to 87.2 μM. Atractylenolide III (3) exhibited selective activity against strain 51 with MIC50 value of 89.9 μM. Both compounds also exhibited anti-inflammatory activity with IC90 values of 23.3 and 31.1 μM, respectively, although they showed little effect on urease. This is the first report on the anti-H. pylori efficacy of various constituents of A. macrocephala and comparative analysis of inhibitory effects against several strains, which will provide scientific evidence supporting its potential as therapeutic agent for H. pylori-related infection.

Keywords: Atractylodes macrocephala; anti-Helicobacter pylori; anti-inflammatory; atractylenolide I; sesquiterpene.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Anti-H. pylori activity of fractions of A. macrocephala.
Figure 2
Figure 2
Compounds isolated from A. macrocephala.
Figure 3
Figure 3
Anti-H. pylori activity of compounds isolated from A. macrocephala. PC: positive control (PC1: quercetin, PC2: metronidazole).
See this image and copyright information in PMC

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