Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Aug 31;39(16):e70839.
doi: 10.1096/fj.202403337RR.

25HC Depleted PMs-Accessible Cholesterol to Restrict SFTSV Entry and Infectious-EVs Mediated Infection

Rui Zhang  1 , Linrun Li  2 , Siyu Li  2 , Shengxia Yin  1 , Min Cheng  2 , Na Jing  2 , Jie Li  1 , Zhiwei Wu  2   3 , Chao Wu  1   4
Affiliations

25HC Depleted PMs-Accessible Cholesterol to Restrict SFTSV Entry and Infectious-EVs Mediated Infection

Rui Zhang et al. FASEB J. .

Abstract

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel tick-borne phlebitis virus of Bunyaviridae, newly named as Banda virus, characterized by high fever, thrombocytopenia, and leukopenia with high case fatality. Currently, no specific antiviral drugs are available to treat patients. Here, we report a natural lipid metabolite 25-Hydroxycholesterol (25HC) that inhibited SFTSV entry by activating the activity of acetyl-CoA-cholesterol acyltransferase (ACAT), a lipid metabolism enzyme, affecting the accessible cholesterol translocation between the cytoplasm and plasma membranes (PMs). We also found that 25HC could inhibit the extracellular vesicles carrying virions into target cells, thus preventing the vesicles from mediating virus spread. In short, 25HC reduced the PMs-accessible cholesterol through modulating lipid metabolism, thereby inhibiting the double infection of cells by virions and infectious EVs, and potentially reducing the efficiency of SFTSV dissemination.

Keywords: 25HC; PMs‐cholesterol; SFTSV; vesicle‐mediated virus transmission; virus entry.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. X. J. Yu, M. F. Liang, S. Y. Zhang, et al., “Fever With Thrombocytopenia Associated With a Novel Bunyavirus in China,” New England Journal of Medicine 364 (2011): 1523–1532, https://doi.org/10.1056/NEJMoa1010095.
    1. T. Takahashi, K. Maeda, T. Suzuki, et al., “The First Identification and Retrospective Study of Severe Fever With Thrombocytopenia Syndrome in Japan,” Journal of Infectious Diseases 209 (2014): 816–827, https://doi.org/10.1093/infdis/jit603.
    1. X. Zhang, Y. Liu, L. Zhao, et al., “An Emerging Hemorrhagic Fever in China Caused by a Novel Bunyavirus SFTSV,” Science China. Life Sciences 56 (2013): 697–700, https://doi.org/10.1007/s11427‐013‐4518‐9.
    1. H. Tani, M. Shimojima, S. Fukushi, et al., “Characterization of Glycoprotein‐Mediated Entry of Severe Fever With Thrombocytopenia Syndrome Virus,” Journal of Virology 90 (2016): 5292–5301, https://doi.org/10.1128/JVI.00110‐16.
    1. M. Spiegel, T. Plegge, and S. Pohlmann, “The Role of Phlebovirus Glycoproteins in Viral Entry, Assembly and Release,” Viruses 8 (2016): 202, https://doi.org/10.3390/v8070202.

LinkOut - more resources