Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Aug 18.
doi: 10.1007/s12011-025-04787-8. Online ahead of print.

Proteomic Insights into Cadmium Resistance of a Novel Levilactobacillus brevis Isolate for Bioremediation Application

Hanane Roula  1 , Houria Ouled-Haddar  2 , Nadjiba Abbas  2 , Mohamed Sifour  2 , Amel Ait-Meddour  2
Affiliations

Proteomic Insights into Cadmium Resistance of a Novel Levilactobacillus brevis Isolate for Bioremediation Application

Hanane Roula et al. Biol Trace Elem Res. .

Abstract

This study aimed to evaluate the cadmium-removal capacity of lactic acid bacteria (LAB) and highlight their potential role in environmental detoxification. LAB strains resistant to cadmium were screened using MRS broth supplemented with cadmium at an initial concentration of 30 mg/L. Out of 50 isolates tested, ten were selected based on their strong growth in the presence of cadmium and their ability to form biofilms. These isolates were identified through 16S rRNA gene sequencing. The isolate N4, identified as Levilactobacillus brevis, exhibited the highest minimum inhibitory concentration (MIC) for cadmium at 600 mg/L and showed effective cadmium removal after 72 h, achieving a removal rate of 36.94% and a cadmium uptake (qe) of 35.415 mg/g. Proteomic analysis of N4 using LC-MS Triple TOF chromatography revealed 1094 proteins. Among these, 65 were unique to the control group, 655 were unique to the cadmium-treated group, and 294 were shared. The differentially abundant proteins were primarily involved in energy metabolism, cell wall synthesis, amino acid and lipid metabolism, and substance transport. These findings enhance our understanding of the molecular mechanisms by which Levilactobacillus brevis binds and removes cadmium, supporting its potential application in mitigating heavy metal pollution.

Keywords: Levilactobacillus brevis; Biofilm; Cd resistance; Environment contamination; Proteomic analysis.

PubMed Disclaimer

Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests.

References

    1. Viard B, Pihan F, Promeyrat S, Pihan JC (2004) Integrated assessment of heavy metal (Pb, Zn, Cd) highway pollution: bioaccumulation in soil, Graminaceae and land snails. Chemosphere 55(10):1349–1359. https://doi.org/10.1016/j.chemosphere.2004.01.003 - DOI - PubMed
    1. Liang J, Feng C, Zeng G, Gao X, Zhong M, Li X, Fang Y (2017) Spatial distribution and source identification of heavy metals in surface soils in a typical coal mine city, Lianyuan, China. Environ Pollut 225:681–690. https://doi.org/10.1016/j.envpol.2017.03.057 - DOI - PubMed
    1. Abbaszade G, Szabó A, Vajna B, Farkas R, Szabó C, Tóth E (2020) Whole genome sequence analysis of Cupriavidus campinensis S14E4C, a heavy metal resistant bacterium. Mol Biol Rep 47:3973–3985. https://doi.org/10.1007/s11033-020-05490-8 - DOI - PubMed - PMC
    1. Zhou YF, Haynes RJ (2010) Sorption of heavy metals by inorganic and organic components of solid wastes: significance to use of wastes as low-cost adsorbents and immobilizing agents. Crit Rev Environ Sci Technol 40(11):909–977. https://doi.org/10.1080/10643380802586857 - DOI
    1. Adepoju-Bello AA, Ojomolade OO, Ayoola GA, Coker HA (2009) Quantitative analysis of some toxic metals in domestic water obtained from Lagos Metropolis. Niger J Pharm 42(1). Retrieved 2023, https://psnnjp.org/index.php/home/article/view/379

LinkOut - more resources