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. 2025 Aug;60(8):e70115.
doi: 10.1111/rda.70115.

Chemical Form of Trace Minerals (Copper, Zinc and Manganese) Fed to Rams Affects Plasma Testosterone Concentrations and Semen Characteristics

Sona Zargari  1 , Armin Towhidi  1 , Kamran Rezayazdi  1 , John Kastelic  2
Affiliations

Chemical Form of Trace Minerals (Copper, Zinc and Manganese) Fed to Rams Affects Plasma Testosterone Concentrations and Semen Characteristics

Sona Zargari et al. Reprod Domest Anim. 2025 Aug.

Abstract

This study investigated the impacts of dietary trace mineral supplementation (copper, zinc and manganese) on plasma testosterone concentrations and semen characteristics in Afshari × Booroola merino rams. Twenty rams were equally allocated into four groups and fed for 70 days, as follows: Control (no supplemental minerals); Sulphate (sulphate forms); Hydroxychloride (30% hydroxychloride, 70% sulphate) and Amino chelate (30% chelate, 70% sulphate). Plasma testosterone concentrations, seminal plasma antioxidant enzyme activities, alkaline phosphatase and total antioxidant capacity were assessed on Days 0 and 70. On Day 70, all these end points were higher (p < 0.05) in the Hydroxychloride and Amino chelate groups compared to the other groups. Furthermore, the Sulphate group had no significant change from Day 0, whereas the Control group decreased (p < 0.05). Ejaculate volume and sperm concentration were measured in fresh semen. Sperm quality was evaluated in fresh and frozen-thawed samples, including motility, morphology, viability and membrane functionality every 14 days. From Day 42, sperm motility, viability and membrane functionality improved (p < 0.05) in the Hydroxychloride and Amino chelate groups. These parameters remained stable in the Sulphate group but declined in the Control group (p < 0.05). Notably, the Control group had the highest percentage of morphologically abnormal sperm at 70 days (p < 0.05). Additionally, the Hydroxychloride and Amino chelate groups had fewer apoptotic sperm and lower malondialdehyde concentrations compared to the Control and Sulphate groups. In conclusion, supplementation with hydroxychloride or amino chelate forms of trace minerals optimised reproductive performance in rams, with higher plasma testosterone concentrations and superior sperm quality compared to sulphate or control diets.

Keywords: chelate; hydroxychloride; ram; semen; sulphate.

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