Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Aug 20.
doi: 10.1007/s12602-025-10725-w. Online ahead of print.

Screening of Lactobacillus plantarum LPH10 and Its Anti-inflammatory Properties: Acid and Bile Salt Tolerance, Bacteriostatic Activity and Regulation of NF-κB/IRF Pathways

Dan Xiong  1   2 , Tao Sun  1   2 , Mengru Liu  1   2 , Bo Wang  1   2 , Tianzhu Guan  1   2 , Wenyuan Zhou  1   2 , Zhenquan Yang  3   4
Affiliations

Screening of Lactobacillus plantarum LPH10 and Its Anti-inflammatory Properties: Acid and Bile Salt Tolerance, Bacteriostatic Activity and Regulation of NF-κB/IRF Pathways

Dan Xiong et al. Probiotics Antimicrob Proteins. .

Abstract

Inflammatory diseases pose a significant threat to human health, and probiotics have increasingly drawn substantial attention for their anti-inflammatory potential. In this research, a model of inflammation was constructed using J774-Dual reporter cells. Lactobacillus plantarum LPH10 with anti-inflammatory properties was screened from Yangzhou pickles. Its characteristics of acid tolerance, bile salt tolerance, bacteriostatic ability, and inhibition of the Salmonella-induced inflammation were evaluated. The results demonstrated that LPH10 exhibited excellent tolerance in simulated gastric acid (pH 3.0) and small intestinal bile salt (0.3%) environments, maintaining high survival rates at both 2 h and 4 h, indicating remarkable acid and bile salt resistance. Bacteriostatic assays demonstrated that LPH10 was effective in restraining the growth of Salmonella enteritidis and Escherichia coli. Moreover, LPH10 displayed sensitivity to a broad spectrum of antibiotics and did not notably affect the viability of RAW264.7 macrophages, which indicated its safety. LPH10 was discovered to be able to potently suppress the activation of the NF-κB and IRF signaling pathways triggered by LPS and S. enteritidis. In the LPS-induced inflammation model, LPH10 downregulated the expression of proinflammatory cytokines such as IL-1β, IL-6, and TNF-α, while upregulating the expression of the anti-inflammatory cytokine IL-10. In the Salmonella-induced inflammation model, LPH10 also regulated the expression levels of inflammatory cytokines, thereby exerting potent anti-inflammatory effects. Overall, L. plantarum LPH10 was successfully screened due to its multiple beneficial properties. This study provides a solid theoretical basis and experimental support for the screening and application of functional anti-inflammatory probiotics and will provide new approaches and probiotic candidates for the development of anti-inflammatory foods and the treatment of inflammatory diseases.

Keywords: Lactobacillus plantarum LPH10; Acid and bile salt tolerance; Anti-inflammation; Bacteriostatic activity; NF-κB/IRF pathways.

PubMed Disclaimer

Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests. Clinical Trial Number: Not applicable.

References

    1. Kim KT, Kim JW, Kim SI, Kim S, Nguyen TH, Kang CH (2021) Antioxidant and anti-inflammatory effect and probiotic properties of lactic acid bacteria isolated from canine and feline feces. Microorganisms 9(9):1971. https://doi.org/10.3300/microorganisms09091971 - DOI - PubMed - PMC
    1. Lee ES, Song EJ, Nam YD, Lee SY (2018) Probiotics in human health and disease: from nutribiotics to pharmabiotics. J Microbiol 56(11):773–782. https://doi.org/10.1007/s12275-018-8293-y - DOI - PubMed
    1. Lopez-Santamarina A, Gonzalez EG, Lamas A, Mondragon ADC, Regal P, Miranda JM (2021) Probiotics as a possible strategy for the prevention and treatment of allergies. A narrative review. Foods 10(4):701. https://doi.org/10.3390/foods10040701 - DOI - PubMed - PMC
    1. Yu P, Ke C, Guo J, Zhang X, Li B (2020) Lactobacillus plantarum L15 alleviates colitis by inhibiting LPS-mediated NF-κB activation and ameliorates DSS-induced gut microbiota dysbiosis. Front Immunol 11:575173. https://doi.org/10.3389/fimmu.2020.575173 - DOI - PubMed - PMC
    1. Kariyawasam KMGMM, Lee NK, Paik HD (2022) Effect of set-type yoghurt supplemented with the novel probiotic Lantiplantibacillus plantarum 200655 on physicochemical properties and the modulation of oxidative stress-induced damage. Food Sci Biotechnol 32(3):353–360. https://doi.org/10.1007/s10068-022-01201-0 - DOI - PubMed - PMC

LinkOut - more resources