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. 1985 Oct;79(5):507-12.
doi: 10.1080/00034983.1985.11811955.

A simple method for the identification of compounds which inhibit tubulin polymerization in filarial worms

R E Howells, C J Delves

A simple method for the identification of compounds which inhibit tubulin polymerization in filarial worms

R E Howells et al. Ann Trop Med Parasitol. 1985 Oct.

Abstract

The incubation in vitro of excised ovaries of Dirofilaria immits in medium containing mebendazole between 10(-5) and 10(-8) M for four or six hours results in the accumulation of up to 20% of oogonial cells in arrested mitotic metaphase. In aceto-orcein-stained squashes of the tissue, cells possess condensed chromosomes but no detectable spindle microtubules. Similar results were obtained with colchicine, but the lowest effective concentration of this drug was 10(-7) M. This procedure affords a simple and rapid method for detecting compounds capable of inhibiting tubulin polymerization in filarial worms.

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