Detection of ESBL-producing Escherichia coli in poultry from Tamaulipas, Mexico

Abstract

The emergence of antibiotic-resistant bacteria has increased, making it difficult to treat infections that are associated with increasing morbidity and mortality. The presence of strains resistant to several antibiotics, such as ESBL-producing Escherichia coli (ESBL-EC), in livestock has been reported in several countries, posing a potential risk to consumer health. Therefore, the objective of this study is to evaluate the prevalence and characteristics of ESBL-producing E. coli in poultry in Tamaulipas, Mexico. Poultry cloacal samples were taken for the identification of ESBL-EC, antibiotic susceptibility patterns were determined, the virulence genes (stx1, stx2 and hlyA) and classification of phylogroups were detected by PCR. The results showed an average prevalence of 17.5% (28/160) ESBL-EC strains in poultry. All strains (28/28) were resistant to ampicillin and ceftriaxone. On the contrary, all strains were sensitive to amikacin, netilmicin, and nitrofurantoin. A total of 64.2% (18/28) of strains were MDR. The 32.1% (9/28) of the strains belonged to the B2 and D phylogroups, which are considered pathogenic groups, with 33.3% (3/9) MDR. This indicates that poultry in Ciudad Victoria, Tamaulipas, is a reservoir of pathogenic strains with antibiotic resistance and MDR, which may pose a risk to public health.

Keywords: ESBL; phylogroups; poultry; resistance; virulence.