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. 2025 Oct:88:103808.
doi: 10.1016/j.scr.2025.103808. Epub 2025 Aug 19.

Generation of two Betacellulin CRISPR-Cas9 knockout hiPSC lines to study the affected EGF system paradigm in schizophrenia

Agustin Cota-Coronado  1 , Murray Manning  2 , Dong-Hyung Kim  3 , Joohyung Lee  4 , Andrew Gibbons  3 , Joseph Rosenbluh  2 , Rachel A Hill  3 , Suresh Sundram  5
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Free article

Generation of two Betacellulin CRISPR-Cas9 knockout hiPSC lines to study the affected EGF system paradigm in schizophrenia

Agustin Cota-Coronado et al. Stem Cell Res. 2025 Oct.
Free article

Abstract

Several members of the epidermal growth factor (EGF) family have been implicated in the biology of schizophrenia (Ketharanathan et al., 2024). The EGF-related ligand, Betacellulin (BTC), plays an important role in the proliferation and differentiation of neural stem cells and our group found markedly reduced BTC levels in patients with schizophrenia. Nevertheless, the interplay of affected BTC and its participation in neural specification and neurodevelopment remains elusive. We generated Knockout (KO) - BTC clones from an existing hiPSC line through CRISPR/Cas9-mediated modification. Furthermore, we validated BTC-KO through genotyping/sequencing, FACS and Western Blot. Finally, we demonstrated trilineage differentiation potential in vitro.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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