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. 2025 Jan;3(1):2-3.
doi: 10.1038/s44222-024-00265-3. Epub 2024 Dec 5.

Developing sex-accurate cell culture environments

Alexandra N Borelli  1 , Kristyn S Masters  1
Affiliations

Developing sex-accurate cell culture environments

Alexandra N Borelli et al. Nat Rev Bioeng. 2025 Jan.

Abstract

The extracellular matrix remains under-recognized as a sex-dependent entity. Designing culture environments that account for sex as a biological variable requires considering not only cellular sex, but also the usage of sex-specific scaffolds to create a holistically sex-accurate platform.

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Conflict of interest statement

Competing interests The authors declare no competing interests.

References

    1. James BD, Guerin P & Allen JB Let’s talk about sex—biological sex is underreported in biomaterial studies. Adv. Healthc. Mater 10, e2001034 (2021). - PMC - PubMed
    1. Walker CJ, Schroeder ME, Aguado BA, Anseth KS & Leinwand LA Matters of the heart: cellular sex differences. J. Mol. Cell. Cardiol 160, 42–55 (2021). - PMC - PubMed
    1. Hernandez PA et al. Sexual dimorphism in the extracellular and pericellular matrix of articular cartilage. Cartilage 10.1177/19476035221121792 (2022). - DOI - PMC - PubMed
    1. Grilo GA et al. Age- and sex-dependent differences in extracellular matrix metabolism associate with cardiac functional and structural changes. J. Mol. Cell. Cardiol 139, 62–74 (2020). - PMC - PubMed
    1. Lock R et al. A framework for developing sex-specific engineered heart models. Nat. Rev. Mater 7, 295–313 (2022). - PMC - PubMed

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