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. 1985 Nov-Dec;68(6):1131-3.

Liquid chromatographic determination of strychnine in stomach contents

J J Hoogenboom, C G Rammell
  • PMID: 4086438

Liquid chromatographic determination of strychnine in stomach contents

J J Hoogenboom et al. J Assoc Off Anal Chem. 1985 Nov-Dec.

Abstract

A chloroform extract of stomach contents at basic pH is concentrated and then extracted with 0.1 M phosphoric acid. The acid extract is chromatographed on a 10 cm reverse phase column, using 0.005 M phosphate buffer (pH 3.0)-acetonitrile-tetrahydrofuran (750 + 135 + 115) containing 0.01 M octanesulfonic acid at a flow rate of 1.0 mL/min for elution. Strychnine eluted in 7.3 min. Recoveries from spiked stomach contents averaged 92%. The method can be used without modification for other alkaloids.

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