Individual Genomic Loci, Transcript Levels, and Serum Profiles of Immune and Antioxidant Markers Associated with Bacteria-Induced Abortion in Sheep (Ovis aries)

Attia Eissa¹, Ahmed A Elsayed², Amin Tahoun^{3

4}, Adel M El-Kattan², Islam M Wassif², Amani A Hafez², Ragab Mohamed⁵, Ahmed I Ateya⁶, Hanan M Alharbi⁷, Khairiah M Alwutayd⁷, Aiman A Ammari⁸, Manal A Babaker⁹, Mansour A Alghamdi^{10

11}, Torsten Bohn¹², Ammar Al-Farga¹³, Hossam M Aljawdah¹⁴

Affiliations

¹ Department of Animal Medicine (Internal Medicine), Faculty of Veterinary Medicine, Arish University, Arish 45511, Egypt.
² Department of Animal Health and Poultry, Animal and Poultry Production Division, Desert Research Center (DRC), Cairo 11753, Egypt.
³ Department of Clinical Veterinary Medical Sciences, Jordan University of Science and Technology, Irbid 302022110, Jordan.
⁴ Department of Animal Medicine, Faculty of Veterinary Medicine, Kafrelshkh University, Kafrelsheikh 33516, Egypt.
⁵ Department of Theriogenology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt.
⁶ Department of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
⁷ Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
⁸ Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
⁹ Department of Chemistry, Faculty of science, Majmaah University, Al Majmaah 11952, Saudi Arabia.
¹⁰ Department of Anatomy, College of Medicine, King Khalid University, Abha 62529, Saudi Arabia.
¹¹ Genomics and Personalized Medicine Unit, The Center for Medical and Health Research, King Khalid University, Abha 62529, Saudi Arabia.
¹² Nutrition and Health Research Group, Population Health Department, Luxembourg Institute of Health, 1A-B, Rue Thomas Edison, L-1445 Strassen, Luxembourg.
¹³ Department of Biological Sciences, College of Science, University of Jeddah, Jeddah 21589, Saudi Arabia.
¹⁴ Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

PMID: 40872670
PMCID: PMC12390017
DOI: 10.3390/vetsci12080719

Individual Genomic Loci, Transcript Levels, and Serum Profiles of Immune and Antioxidant Markers Associated with Bacteria-Induced Abortion in Sheep (Ovis aries)

Attia Eissa et al. Vet Sci. 2025.

. 2025 Jul 31;12(8):719.

doi: 10.3390/vetsci12080719.

Authors

Affiliations

¹ Department of Animal Medicine (Internal Medicine), Faculty of Veterinary Medicine, Arish University, Arish 45511, Egypt.
² Department of Animal Health and Poultry, Animal and Poultry Production Division, Desert Research Center (DRC), Cairo 11753, Egypt.
³ Department of Clinical Veterinary Medical Sciences, Jordan University of Science and Technology, Irbid 302022110, Jordan.
⁴ Department of Animal Medicine, Faculty of Veterinary Medicine, Kafrelshkh University, Kafrelsheikh 33516, Egypt.
⁵ Department of Theriogenology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt.
⁶ Department of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
⁷ Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
⁸ Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
⁹ Department of Chemistry, Faculty of science, Majmaah University, Al Majmaah 11952, Saudi Arabia.
¹⁰ Department of Anatomy, College of Medicine, King Khalid University, Abha 62529, Saudi Arabia.
¹¹ Genomics and Personalized Medicine Unit, The Center for Medical and Health Research, King Khalid University, Abha 62529, Saudi Arabia.
¹² Nutrition and Health Research Group, Population Health Department, Luxembourg Institute of Health, 1A-B, Rue Thomas Edison, L-1445 Strassen, Luxembourg.
¹³ Department of Biological Sciences, College of Science, University of Jeddah, Jeddah 21589, Saudi Arabia.
¹⁴ Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

PMID: 40872670
PMCID: PMC12390017
DOI: 10.3390/vetsci12080719

Abstract

Aboudeleik sheep are a prominent native breed in desert and semi-desert areas, known for their adaptability to harsh climates and their utility in both milk and meat production [...].

Keywords: abortion and sheep; antioxidants; gene expression; immunity; single-nucleotide polymorphisms.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

**Figure 1**
(A–C) Electrophoresis analysis of PCR product of amplified (A) *Brucella melitensis*, (B) *Salmonella* spp., and (C) *Campylobacter* spp. (A) Lanes 1, 2, 3, and 5 indicate a positive amplification of *Brucella melitensis* at the 839 bp, (B) Lanes 1, 2, and 4 indicate positive amplification of *Salmonella* at the 284 bp, and (C) all samples indicate positive amplification at 650 bp for *Campylobacter*.

**Figure 2**
(A–D) Electrophoresis analysis of PCR product of amplified (A) *Coxiella burnetii*, (B) *Chlamydia psittaci*, (C) *Listeria monocytogens,* and (D) *Leptospira*. All sample negative for all bacteria at 687, 300, 1200 and 202 bp, respectively.

**Figure 3**
Comparative analysis of immune-related genes in normal and abortion-affected ewes. Values are mean ± SE. The symbol * denotes statistical significance (* p < 0.05).

**Figure 4**
Comparative analysis of antioxidant gene expression in healthy and abortion-affected ewes. Values are mean ± SE. Asterisks (*) indicate statistically significant differences (* p < 0.05).

See this image and copyright information in PMC

References

1. March Desert Research Center . Development of Animal Wealth in Shalateen, Aburamad and Halaieb. Volume 17. March Desert Research Center; Cairo, Egypt: 1996. p. 7. First Progress Report.
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1. Ali S., Zhao Z.-H., Zhen G., Kang J.Z., Yi P.Z. Reproductive problems in small ruminants (sheep and goats): A substantial economic loss in the world. Large Anim. Rev. 2019;25:215–223.
1. Lokamar P.N., Kutwah M.A., Atieli H., Gumo S., Ouma C. Socio-economic impacts of brucellosis on livestock production and reproduction performance in Koibatek and Marigat regions, Baringo County, Kenya. BMC Vet. Res. 2020;16:61. doi: 10.1186/s12917-020-02283-w. - DOI - PMC - PubMed
1. Alemayehu G., Mamo G., Alemu B., Desta H., Tadesse B., Benti T., Bahiru A., Yimana M., Wieland B. Causes and flock level risk factors of sheep and goat abortion in three agroecology zones in Ethiopia. Front. Vet. Sci. 2021;8:615310. doi: 10.3389/fvets.2021.615310. - DOI - PMC - PubMed

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Individual Genomic Loci, Transcript Levels, and Serum Profiles of Immune and Antioxidant Markers Associated with Bacteria-Induced Abortion in Sheep (Ovis aries)

Affiliations

Individual Genomic Loci, Transcript Levels, and Serum Profiles of Immune and Antioxidant Markers Associated with Bacteria-Induced Abortion in Sheep (Ovis aries)

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

References

Grants and funding

LinkOut - more resources

Full Text Sources