[DMNH--a new competent indicator for measuring the chemiluminescence of neutrophilic granulocytes and monocytes]

Abstract

Comparative studies on the effects of the luminescence indicators 5-Amino-2,3-dihydro-1,4-phthalazinedione (Luminol, LUM) and 7-Dimethylamino-naphthalene-1,2-dicarbonic-acidhydracide (DMNH) in measuring the chemiluminescence of ingesting and non-ingesting polymorphonuclear leukocytes (PMN) and monocytes (MOC) are described. DMNH is more effective than LUM in measuring chemiluminescence of PMN and MOC under both biological conditions. Indications are given that LUM inhibits the oxidative metabolism of both cell types. The use of DMNH as a luminescence indicator makes possible the research of cells with low activity of the oxidative metabolism in small numbers.