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. 2025 Aug 29;74(1):115.
doi: 10.1007/s00011-025-02083-8.

Impact of sepsis on bone marrow mesenchymal stem cells and its implications for hematopoiesis and immunosuppression

Thatiana Correa de Melo  1 , Suely Kunimi Kubo Ariga  2 , Thais Martins de Lima  2 , Debora Levy  3 , Sérgio Paulo Bydlowski  3 , Francisco Garcia Soriano  4
Affiliations

Impact of sepsis on bone marrow mesenchymal stem cells and its implications for hematopoiesis and immunosuppression

Thatiana Correa de Melo et al. Inflamm Res. .

Abstract

Objective and design: Septic patients often exhibit disruption of the normal hematopoiesis, leading to hematological abnormalities such as anaemia, leukopenia, and thrombocytopenia. We hypothesized that sepsis-induced changes in bone marrow mesenchymal stromal cells (BM-MSCs) contribute to the abnormal hematopoiesis observed in these patients.

Material and methods: We established lineages of BM-MSCs from male BALB/c mice collected 8 h after the sham (MSC-CT) or cecal ligation and puncture surgery (MSC-Sepsis). We evaluated BM-MSCs proliferation, plasticity, and immunomodulatory properties.

Results: No differences in multipotency or immunophenotypic profiles were detected between the MSC-CT and MSC-Sepsis groups. However, MSC plasticity was clearly affected by sepsis, as osteoblast differentiation was impaired in MSC-Sepsis. Since differentiation capacity is closed linked to mitochondrial dynamics and function, we assessed mitochondrial health and found that MSC-Sepsis presented depolarized mitochondria. The photoelectron micrographs supported these findings, as MSC-Sepsis presented higher number of small mitochondria around the nuclei and deformed cristae in the mitochondria. Additionally, cytokine array analysis revealed a marked reduction in the expression of several cytokines and chemokines in MSC-Sepsis.

Conclusion: Our findings demonstrate that sepsis induces several functional alterations in MSC that may impair bone marrow homeostasis and contribute to both the acute immune response and long-term complications in sepsis survivors.

Keywords: Bone marrow mesenchymal stromal cells; Hematopoiesis; Immunosuppression; Mitochondria; Sepsis.

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Conflict of interest statement

Declarations. Conflict of interest: The authors declare no competing interests. Ethics approval and consent to participate: All procedures were performed according to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health. The study protocol was approved by the Research Ethics Committee of the School of Medicine – USP (# 0289/12).

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References

    1. Jarczak D, Kluge S, Nierhaus A. Sepsis—pathophysiology and therapeutic concepts. Front Med. 2021;8: 628302.
    1. Barbeiro DF, Barbeiro HV, Faintuch J, Ariga SKK, Mariano M, Popi AF, et al. B-1 cells temper endotoxemic inflammatory responses. Immunobiology. 2011;216:302–8. - PubMed
    1. da-Silva FP, Chiamolera M, Charles N, Kanamaru Y, Velasco IT, Benhamou M, et al. B lymphocytes undergo apoptosis because of FcγRIIb stress response to infection: a novel mechanism of cell death in sepsis. Shock. 2006;25:61–5.
    1. Hotchkiss RS, Tinsley KW, Swanson PE, Schmieg RE, Hui JJ, Chang KC, et al. Sepsis-induced apoptosis causes progressive profound depletion of B and CD4+ T lymphocytes in humans. J Immunol. 2001;166:6952–63. - PubMed
    1. Zaretsky AG, Engiles JB, Hunter CA. Infection-induced changes in hematopoiesis. J Immunol. 2014;192:27–33.

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