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. 2025 Aug 1;8(8):e2529837.
doi: 10.1001/jamanetworkopen.2025.29837.

Dried Blood Spot PCR for Detection of Congenital Cytomegalovirus Infection and Disease

Mark R Schleiss  1 , Erin A Osterholm  2 , Ryan Shanley  3 , Nelmary Hernandez-Alvarado  1 , Emily Graupmann  1 , Rebecca Kruc  1 , Rick Jansen  4 , Melissa Polonenko  5 , Hannah Herd  6 , Claudia Fernandez-Alarcon  1 , Mark Blackstad  1 , Sondra Rosendahl  7 , Kirsten Coverstone  7 , Mark McCann  7 , Abbey Sidebottom  8 , Whitney Wunderlich  8 , Sasha Strul  9 , Kathleen A Kulus  10 , Sheila C Dollard  11 , Tatiana M Lanzieri  11
Affiliations

Dried Blood Spot PCR for Detection of Congenital Cytomegalovirus Infection and Disease

Mark R Schleiss et al. JAMA Netw Open. .
No abstract available

Plain language summary

This cohort study evaluates the performance of dried blood spot polymerase chain reaction (PCR) testing for the identification of congenital cytomegalovirus among infants in Minnesota.

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Conflict of interest statement

Conflict of Interest Disclosures: Dr Schleiss reported serving as site principal investigator for vaccine studies funded by Moderna Vaccines at the University of Minnesota; receiving grants from the US Centers for Disease Control and Prevention (CDC), the National Institutes of Health, and the University of South Carolina Disability Research and Dissemination Center (DRDC) during the conduct of the study; and receiving personal fees from Moderna, Sanofi, GlaxoSmithKline, and Merck outside the submitted work. Dr Jansen reported receiving grants from CDC during the conduct of the study. Dr Polonenko reported receiving grants from the National Institute on Deafness and Other Communication Disorders subaward to the University of Minnesota and the Hearing Health Foundation outside the submitted work. Dr Herd reported receiving salary support from the National Institutes of Health for serving as a site principal investigator; receiving grants from the Minnesota Lions Hearing Foundation; and serving on the advisory committee of the Minnesota Department of Health Newborn Hearing Screening and on the membership development committee of the Minnesota Academy of Audiology. Dr Rosendahl reported receiving grants from the CDC Emerging Infections Program (EIP) during the conduct of the study. Dr Coverstone reported receiving grants from the CDC EIP during the conduct of the study. Dr Sidebottom reported receiving grants from the CDC during the conduct of the study. Dr Kulus reported receiving grants from the Minnesota Department of Health, which paid for supplies and the salary for a research assistant, during the conduct of the study. No other disclosures were reported.

References

    1. Lutz CS, Schleiss MR, Fowler KB, Lanzieri TM. Updated national and state-specific prevalence of congenital cytomegalovirus infection, United States, 2018-2022. J Public Health Manag Pract. 2025;31(2):234-243. doi: 10.1097/PHH.0000000000002043 - DOI - PMC - PubMed
    1. Kaye T, Dufort EM, Rosendahl SD, et al. Notes from the field: universal newborn screening and surveillance for congenital cytomegalovirus—Minnesota, 2023-2024. MMWR Morb Mortal Wkly Rep. 2024;73(32):703-705. doi: 10.15585/mmwr.mm7332a2 - DOI - PubMed
    1. Dollard SC, Dreon M, Hernandez-Alvarado N, et al. Sensitivity of dried blood spot testing for detection of congenital cytomegalovirus infection. JAMA Pediatr. 2021;175(3):e205441. doi: 10.1001/jamapediatrics.2020.5441 - DOI - PMC - PubMed
    1. Rawlinson WD, Boppana SB, Fowler KB, et al. Congenital cytomegalovirus infection in pregnancy and the neonate: consensus recommendations for prevention, diagnosis, and therapy. Lancet Infect Dis. 2017;17(6):e177-e188. doi: 10.1016/S1473-3099(17)30143-3 - DOI - PubMed
    1. Schleiss MR, Rosendahl S, McCann M, Dollard SC, Lanzieri TM. Assessment of congenital cytomegalovirus prevalence among newborns in Minnesota during the COVID-19 pandemic. JAMA Netw Open. 2022;5(9):e2230020. doi: 10.1001/jamanetworkopen.2022.30020 - DOI - PMC - PubMed