Studies on the distribution of the antigens detected by some newly prepared monoclonal antibodies in normal hemopoietic and leukemic cells

Abstract

A great number of hybridoma clones has been developed in two independent fusions after immunization with cells of a human non-T, non-B REH leukemic cell line, recognizing different differentiation antigens on leukocytes. Only four of these new murine monoclonal hybridoma-derived antibodies (Bra20, Bra30, BraFB6 and BraEA10) have been used in our study for determination of their reactivity spectrum in normal blood cells and fresh lymphoid and non-lymphoid leukemic cells. The resulting reactivity spectra of these four monoclonal antibodies in normal hemopoietic and leukemic cells in indirect immunofluorescence assay are in agreement with supposed Ia-like reactivity for Bra20, Bra30 and Bra FB6 monoclonal antibodies and "common" leukocyte specificity for BraEA10 monoclonal antibody.