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. 1976 Apr 6;15(7):1481-9.
doi: 10.1021/bi00652a019.

Optimal conditions and specificity of interaction of a distinct class of nonhistone chromosomal proteins with DNA

T L Thomas, G L Patel

Optimal conditions and specificity of interaction of a distinct class of nonhistone chromosomal proteins with DNA

T L Thomas et al. Biochemistry. .

Abstract

A subclass of nonhistone chromatin proteins with high DNA affinity has been isolated from rat liver. The interaction of the isolated proteins with DNA in vitro was characterized utilizing a nitrocellulose filter binding technique. The temperature, time, concentration, ionic strength, and pH dependence were characterized. Optimal interaction was observed at 0.19 M naCl, pH 7.5 with a protein to DNA ratio of 13 (w/w). Equilibrium and kinetic competition experiments indicated that these proteins interact optimally with A-T rich and single-stranded DNA. The data also suggest that these proteins might affect the helixcoil transiton of DNA.

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