Simultaneous demonstration of cholinesterases and glyoxylic acid-induced fluorescence of catecholamines in stretch preparations
- PMID: 409035
- DOI: 10.1016/S0065-1281(77)80128-1
Simultaneous demonstration of cholinesterases and glyoxylic acid-induced fluorescence of catecholamines in stretch preparations
Abstract
A combined simultaneous method to demonstrate adrenergic nerves using glyoxylic acid-induced fluorescence and nerves showing cholinesterase activity using the thiocholine technique is described in whole-mount preparations. The subcutaneous fascia and the right atrium of the heart of the rat and guinea-pig were used as tissue specimens, and the innervation patterns of adrenergic and cholinergic nerves were demonstrated in UV and transmitted light. Technical points and the limitations of the method are discussed.
Similar articles
-
Simultaneous and consecutive histochemical demonstration of glyoxylic acid-induced fluorescence of catecholamines and cholinesterase reactions.Cell Mol Biol Incl Cyto Enzymol. 1978;23(3):215-24. Cell Mol Biol Incl Cyto Enzymol. 1978. PMID: 737670 No abstract available.
-
Demonstration of catecholamines in peripheral adrenergic nerves in stretch preparations with fluorescence induced by aqueous solution of glyoxylic acid.Histochemistry. 1975;41(4):369-72. doi: 10.1007/BF00490080. Histochemistry. 1975. PMID: 1141023
-
Simultaneous demonstration of catecholamines and acetylcholinesterase in peripheral autonomic nerves.Am J Anat. 1972 Sep;135(1):23-32. doi: 10.1002/aja.1001350104. Am J Anat. 1972. PMID: 4560620 No abstract available.
-
The histochemical fluorescence method for the demonstration of catecholamines. Theory, practice and application.J Histochem Cytochem. 1973 Apr;21(4):293-311. doi: 10.1177/21.4.293. J Histochem Cytochem. 1973. PMID: 4574996 Review. No abstract available.
-
Histochemistry of nervous tissues: catecholamines and cholinesterases.Annu Rev Pharmacol. 1967;7:203-22. doi: 10.1146/annurev.pa.07.040167.001223. Annu Rev Pharmacol. 1967. PMID: 5337080 Review. No abstract available.
Cited by
-
Congruence of adrenergic and cholinergic intrinsic innervation of human and rat atria.Basic Res Cardiol. 1987 Sep-Oct;82(5):445-53. doi: 10.1007/BF01907092. Basic Res Cardiol. 1987. PMID: 3426523
-
Consecutive demonstration of catecholamines and dopamine-beta-hydroxylase within the same specimen.Histochemistry. 1979 Jul 11;61(3):301-5. doi: 10.1007/BF00508451. Histochemistry. 1979. PMID: 383661
MeSH terms
Substances
LinkOut - more resources
Full Text Sources