doi: 10.1016/0003-2697(85)90533-0.
DNA methyltransferases: activity minigel analysis and determination with DNA covalently bound to a solid matrix
- PMID: 4091268
- DOI: 10.1016/0003-2697(85)90533-0
Item in Clipboard
DNA methyltransferases: activity minigel analysis and determination with DNA covalently bound to a solid matrix
Anal Biochem.
.
Abstract
We describe two methods that facilitate detection and characterization of DNA methyltransferases: activity gel analysis and the use of DNA-cellulose or DNA-Sepharose in DNA methylation reactions. The first permits identification of catalytic subunits, determination of the influence of proteolysis, and evolutionary or developmental studies. The second allows accurate and fast determination of DNA methyltransferase activities in crude extracts and during purification.
Similar articles
-
De novo and maintenance DNA methylation by a mouse plasmacytoma cell DNA methyltransferase.J Biol Chem. 1988 Mar 25;263(9):4392-9. J Biol Chem. 1988. PMID: 3346252
-
Isolation and characterization of proteins that stimulate the activity of mammalian DNA methyltransferase.Biochim Biophys Acta. 1988 Nov 10;951(1):201-12. doi: 10.1016/0167-4781(88)90041-3. Biochim Biophys Acta. 1988. PMID: 3142521
-
DNA methylation in wheat. Purification and properties of DNA methyltransferase.Eur J Biochem. 1987 Aug 17;167(1):89-96. doi: 10.1111/j.1432-1033.1987.tb13307.x. Eur J Biochem. 1987. PMID: 3622512
-
DNA methyltransferases.Curr Opin Cell Biol. 1994 Jun;6(3):380-9. doi: 10.1016/0955-0674(94)90030-2. Curr Opin Cell Biol. 1994. PMID: 7917329 Review.
-
[Dnmt2 is the Most Evolutionary Conserved and Enigmatic Cytosine DNA Methyltransferase in Eukaryotes].Genetika. 2016 Mar;52(3):269-82. Genetika. 2016. PMID: 27281847 Review. Russian.
Cited by
-
A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding.Nucleic Acids Res. 2009 Feb;37(3):e22. doi: 10.1093/nar/gkn1029. Epub 2009 Jan 7. Nucleic Acids Res. 2009. PMID: 19129216 Free PMC article.
-
The amino acid sequence of the CCGG recognizing DNA methyltransferase M.BsuFI: implications for the analysis of sequence recognition by cytosine DNA methyltransferases.EMBO J. 1990 Apr;9(4):1007-13. doi: 10.1002/j.1460-2075.1990.tb08203.x. EMBO J. 1990. PMID: 2108858 Free PMC article.
-
Multiple domains are involved in the targeting of the mouse DNA methyltransferase to the DNA replication foci.Nucleic Acids Res. 1998 Feb 15;26(4):1038-45. doi: 10.1093/nar/26.4.1038. Nucleic Acids Res. 1998. PMID: 9461465 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
