Combination of neovestitol and vestitol impair the subgingival multispecies biofilm development

Abstract

The aim of this study was to evaluate effects of neovestitol-vestitol fraction (NVF) on an in vitro subgingival multispecies biofilm. The 33-species biofilm was formed for seven days using a Calgary device. Starting on day 3, treatments for applied twice daily for 1 min each: NV (400-1,600 µg ml^-1), chlorhexidine 0.12% (CHX; positive control) or vehicle (negative control). After seven days, metabolic activity and microbial composition were accessed through colorimetric reaction and DNA-DNA hybridization, respectively. ANOVA/Tukey's and Kruskal-Wallis/Dunn's were performed (p < 0.05). NV1,600 and NV800 and CHX significantly reduced biofilm metabolic activity by 67%, 48% and 64% respectively, compared to vehicle-treatment. NV1,600, NV800 and CHX reduced red complex proportions versus vehicle-treatment. NV1,600 also reduced orange complex and increased healthy-associated purple complex compared to negative control (p < 0.05). NV1,600, NV800 and CHX reduced nine species, including Fusobacterium periodonticum and Porphyromonas gingivalis. NV1,600 also reduced Fusobacterium nucleatum polymorphum. NV seems to be a good candidate to control biofilm formation and pathogenicity in dental practice.

Keywords: Subgingival biofilm; antimicrobial; neovestitol; periodontitis; red propolis; vestitol.