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. 1985 Dec 1;232(2):467-70.
doi: 10.1042/bj2320467.

Purification of fish muscle myosin heavy chain and quantification of the specific polyribosome-bound polypeptide

E Lied, A von der Decken

Purification of fish muscle myosin heavy chain and quantification of the specific polyribosome-bound polypeptide

E Lied et al. Biochem J. .

Abstract

A purification procedure for fish myosin heavy chain is described. The protein was injected into rabbits for production of antibodies. The specificity of the antibodies was determined by immunoblotting. The enzyme-linked immunosorbent assay technique was applied to quantify myosin heavy chain bound to isolated polyribosomes of epaxial muscle from fish.

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