A DNA template recognition protein: partial purification from mouse liver and stimulation of DNA polymerase alpha
- PMID: 4092024
- DOI: 10.1021/bi00347a007
A DNA template recognition protein: partial purification from mouse liver and stimulation of DNA polymerase alpha
Abstract
A protein that specifically enhances up to 13-fold the rate of copying of poly(dT) template by DNA polymerase alpha was partially purified from chromatin of regenerating mouse liver cells. This stimulatory protein, designated herein factor D, also increases 2-3-fold the activity of polymerase alpha with heat-denatured DNA and with primed, circular single-stranded phi X174 DNA. However, factor D has no detectable effect on the copying by polymerase alpha of poly(dG), poly(dA), and poly(dC) templates. Activity of mouse DNA polymerase beta is not affected by factor D with all the tested templates. In contrast to polymerase alpha, factor D is resistant to inactivation by N-ethylmaleimide and calcium ions, but it is readily heat-inactivated at 46 degrees C and is inactivated by trypsin digestion. Partially purified factor D is not associated with detectable activities of DNA polymerase, DNA primase, deoxyribonucleotidyl terminal transferase, and endo- or exodeoxyribonuclease.
Similar articles
-
Template-selective stimulation of diverse DNA polymerases by the murine DNA-binding protein factor D.J Biol Chem. 1987 Jun 25;262(18):8861-7. J Biol Chem. 1987. PMID: 3597397
-
Factor C from rabbit liver. A new poly(dC) and poly[d(G-C)] template-selective stimulatory protein of DNA polymerases.J Biol Chem. 1989 Mar 25;264(9):5245-52. J Biol Chem. 1989. PMID: 2925691
-
DNA polymerase alpha cofactors C1C2 function as primer recognition proteins.J Biol Chem. 1983 Aug 25;258(16):9810-9. J Biol Chem. 1983. PMID: 6224785
-
HeLa DNA polymerase alpha activity in vitro: specific stimulation by a non-enzymic protein factor.Nucleic Acids Res. 1978 Jan;5(1):221-39. doi: 10.1093/nar/5.1.221. Nucleic Acids Res. 1978. PMID: 643610 Free PMC article.
-
Rabbit liver factor D, a poly(thymidine) template stimulatory protein of DNA polymerases: purification and characterization.Biochemistry. 1988 Apr 19;27(8):2990-7. doi: 10.1021/bi00408a049. Biochemistry. 1988. PMID: 3401461
Cited by
-
Factor D is a selective single-stranded oligodeoxythymidine binding protein.Nucleic Acids Res. 1988 Jan 11;16(1):199-211. doi: 10.1093/nar/16.1.199. Nucleic Acids Res. 1988. PMID: 3340524 Free PMC article.
-
The AT-rich tract of the SV40 ori core: negative synergism and specific recognition by single stranded and duplex DNA binding proteins.Nucleic Acids Res. 1992 Jul 11;20(13):3333-9. doi: 10.1093/nar/20.13.3333. Nucleic Acids Res. 1992. PMID: 1321411 Free PMC article.
-
Sequence recognition protein for the 17-base-pair A + T-rich tract in the replication origin of simian virus 40 DNA.Proc Natl Acad Sci U S A. 1989 Jan;86(1):70-4. doi: 10.1073/pnas.86.1.70. Proc Natl Acad Sci U S A. 1989. PMID: 2536162 Free PMC article.