Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Sep 10.
doi: 10.1007/s00210-025-04470-8. Online ahead of print.

In silico design, synthesis of a novel chalcone derivative and its in vitro and in vivo anticancer activity against colon cancer-induced mouse model

Uday Raj Sharma  1 , Sushavan Roy  2 , Gurubasavaraja Swamy Purawargaar Matada  3 , Manjunatha P M  2 , Suresh Janadri  2 , Surendra Vada  2 , T Haribabu  2 , Nageena Taj  2 , Gayathri S V  2 , Rohit Pal  2 , Susovan Das  2 , Nirmal Chanda  2
Affiliations

In silico design, synthesis of a novel chalcone derivative and its in vitro and in vivo anticancer activity against colon cancer-induced mouse model

Uday Raj Sharma et al. Naunyn Schmiedebergs Arch Pharmacol. .

Abstract

This study aimed to synthesize and evaluate the anticancer activity of novel chalcone derivative against colon cancer by in vitro cytotoxicity against HCT-116 (Research Resource Identifiers:CVCL_D4JB) cell line and in vivo using EAC (Research Resource Identifiers: CVCL_1306) and DLA (Research Resource Identifiers: CVCL_VR37) cells inoculated Swiss albino mice. The present study aimed to synthesize the new chalcone derivatives and conduct its anti-colon cancer activity both in vitro and in vivo. The designed compounds were subjected to in silico studies like binding pocket analysis, molecular docking, and ADME studies. The compounds were synthesized using the Claisen-Schmidt condensation technique. The synthesized compounds were then characterized using FT-IR, NMR, and MS. Further, synthesized the compounds were subjected to an in vitro cytotoxicity study using an MTT assay against the HCT-116 cell line. The therapeutic efficacy of novel chalcone derivative was evaluated against (Ehrlich Ascites Carcinoma) EAC and (Dalton's Lymphoma Ascites) DLA cell inoculated Swiss albino mice. The characterization of the compounds using FT-IR, NMR, and MS confirmed the formation of the new chalcone derivatives. One synthesized compound showed good cytotoxicity against the HCT-116 cell line with an IC50 value of 15.90 μg/ml. In the in vivo pharmacodynamic parameters, the S1 maintained stable body weight, decrease in tumour volume, and tumour weight, and increased the life span of cancer-induced mice. Haematological profiles such as RBC, WBC, Hb, and Platelet count revealed nearly normal levels in S1-treated mice in both EAC and DLA cell-inoculated mice. All the observations convey that the novel chalcone derivative (S1) has potency in dose-dependent anticancer activity comparable to 5-flurouracil.

Keywords: Colon cancer; DLA; EAC; HCT-116.

PubMed Disclaimer

Conflict of interest statement

Declarations. Ethical approval: The animal experiments were approved by the Institutional Animal Ethics Committee (IAEC) of Acharya and BM Reddy College of Pharmacy. The study adhered to the guidelines of the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Government of India. Approval Number: [IAEC/ABMRCP/2023–2024/24]. Competing interest: The authors declare no competing interests.

References

    1. Anjali CJ, Pareeth CM, Babu TD (2023) Analysis of cytotoxic and antitumor potential of Passiflora quadrangularis. J Herbs Spices Med Plants 29(4):452–469. https://doi.org/10.1080/10496475.2023.2225147 - DOI
    1. Chandrashekar NS, Prasanth VV (2008) Clinical evaluation of 5-fluorouracil from transdermal patches on EAC and DLA cell-induced tumors in mice. Asian Pac J Cancer Prev APJCP 9:437–40
    1. Dey A, Mitra A, Pathak S, Prasad S, Zhang AS, Zhang H, Sun X-F, Banerjee A (2023) Recent advancements, limitations, and future perspectives of the use of personalized medicine in treatment of colon cancer. Technol Cancer Res Treat 22:153303382311784 - DOI
    1. El-Atawy MA, Hanna DH, Bashal AH, Ahmed HA, Alshammari EM, Hamed EA, Aljohani AR, Omar AZ (2024) Synthesis, characterization, antioxidant, and anticancer activity against colon cancer cells of some cinnamaldehyde-based chalcone derivatives. Biomolecules. https://doi.org/10.3390/BIOM14020216 - DOI - PubMed - PMC
    1. Gaonkar SL, Vignesh UN (2017) Synthesis and pharmacological properties of chalcones: a review. Res Chem Intermed 43:6043–6077 - DOI

LinkOut - more resources