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. 2025 Jun 27:51:59.
doi: 10.11604/pamj.2025.51.59.45565. eCollection 2025.

Multilocus sequence type analysis of coagulase-negative staphylococci from the neonatal intensive care unit of a teaching hospital in Ghana

Innocent Afeke  1 , Joseph Adu-Amankwaah  2 , Ibrahim Jamfaru  3 , Abdul-Wahab Mawuko Hamid  1 , Verner Ndudiri Orish  3 , Bright Agbodzi  4 , Kokou Hefoume Amegan-Aho  5 , Lennox Mac Ankrah  3 , Hintermann Kobina Mbroh  6 , Graceful Lord Mensah  7 , Anthony Samuel Ablordey  4
Affiliations

Multilocus sequence type analysis of coagulase-negative staphylococci from the neonatal intensive care unit of a teaching hospital in Ghana

Innocent Afeke et al. Pan Afr Med J. .

Abstract

Introduction: coagulase-negative staphylococci (CoNS) have emerged as significant pathogens in neonatal intensive care units (NICUs), especially as a leading cause of neonatal sepsis. In low-income countries, efforts to prevent sepsis-related mortality are hampered by a lack of comprehensive molecular data on bacterial isolates responsible for these infections. This study aims to address this gap by generating whole-genome sequencing data, identifying novel multilocus sequence (MLS) types, and investigating antibiotic resistance genes in Staphylococcus epidermidis and Staphylococcus haemolyticus from the NICU of Ho Teaching Hospital in Ghana.

Methods: a total of 123 CoNS isolates were tested for minimum inhibitory concentrations (MIC) of 13 antibiotics using VITEK2, and 16 isolates (7 S. epidermidis and 9 S. haemolyticus) were selected for further genomic analysis. Genomic DNA was extracted and sequenced using an Illumina NovaSeq 6000 platform. Draft genome sequences were assembled using MEGAHIT and annotated with Prokka. MLS types were identified using the Center for Genomic Epidemiology´s MLST tool, and antibiotic resistance genes were detected via the Comprehensive Antibiotic Resistance Database (CARD) and BlastArg-annot Nt tools.

Results: draft genome sequences were established for 7 S. epidermidis and 9 S. haemolyticus isolates and deposited in public databases. Five novel MLST types were discovered: ST89, ST90, and ST91 for S. haemolyticus, and ST993 and ST994 for S. epidermidis. These novel strains exhibited multidrug resistance, with multiple antimicrobial resistance genes identified in their genomes. Three of the newly identified MLST types were isolated from the blood of neonates, while two others were found on the nasal mucosa of clinical staff and a baby cot.

Conclusion: our findings highlight the presence of multidrug-resistant and novel CoNS MLST types in the NICU, suggesting an urgent need for a nationwide database on CoNS MLST types circulating in Ghana. Such data would be critical for developing informed public health policies and strategies to manage neonatal sepsis in the Region.

Keywords: Coagulase-negative staphylococci; antimicrobial resistance; microbial genomics; multilocus sequence analysis; neonatal sepsis.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
coagulase-negative staphylococci (CoNS) multilocus sequence type database A): Staphylococcus haemolyticus B): Staphylococcus epidermidis
See this image and copyright information in PMC

References

    1. Michels R, Last K, Becker SL, Papan C. Update on Coagulase-Negative Staphylococci-What the Clinician Should Know. Microorganisms. 2021 Apr 14;9(4):830. - PMC - PubMed
    1. Eltwisy HO, Twisy HO, Hafez MH, Sayed IM, El-Mokhtar MA. Clinical Infections, Antibiotic Resistance, and Pathogenesis of Staphylococcus haemolyticus. Microorganisms. 2022 May 31;10(6):1130. - PMC - PubMed
    1. França A. The Role of Coagulase-Negative Staphylococci Biofilms on Late-Onset Sepsis: Current Challenges and Emerging Diagnostics and Therapies. Antibiotics (Basel) 2023 Mar 10;12(3):554. - PMC - PubMed
    1. Serra-Burriel M, Keys M, Campillo-Artero C, Agodi A, Barchitta M, Gikas A, et al. Impact of multi-drug resistant bacteria on economic and clinical outcomes of healthcare-associated infections in adults: Systematic review and meta-analysis. PLoS One. 2020 Jan 10;15(1):e0227139. - PMC - PubMed
    1. Hsu LY, Harris SR, Chlebowicz MA, Lindsay JA, Koh TH, Krishnan P, et al. Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system. Genome Biol. 2015 Apr 23;16(1):81. - PMC - PubMed

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