A call for a unified and multimodal definition of cellular identity in the enteric nervous system

Homa Majd^#^{1

2}, Andrius Cesiulis^#^{3

4}, Ryan M Samuel^#^{3

4}, Mikayla N Richter^{3

4}, Nicholas Elder^{3

4}, Kwun Wah Wen⁵, Richard A Guyer⁶, Marlene M Hao⁷, Lincon A Stamp⁷, Allan M Goldstein⁶, Faranak Fattahi^#^{8

9

10}

Affiliations

¹ Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, 94158, USA. Homa.Majd@ucsf.edu.
² Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, 94143, USA. Homa.Majd@ucsf.edu.
³ Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, 94158, USA.
⁴ Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, 94143, USA.
⁵ Department of Pathology, University of California, San Francisco, San Francisco, CA, USA.
⁶ Department of Pediatric Surgery, Massachusetts General Hospital, Boston, MA, USA.
⁷ Department of Anatomy and Physiology, the University of Melbourne, Parkville, VIC, Australia.
⁸ Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, 94158, USA. Faranak.Fattahi@ucsf.edu.
⁹ Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, 94143, USA. Faranak.Fattahi@ucsf.edu.
¹⁰ Program in Craniofacial Biology, University of California, San Francisco, CA, USA. Faranak.Fattahi@ucsf.edu.

^# Contributed equally.

PMID: 40954253
DOI: 10.1038/s44318-025-00559-1

Free article

A call for a unified and multimodal definition of cellular identity in the enteric nervous system

Homa Majd et al. EMBO J. 2025.

Free article

. 2025 Sep 15.

doi: 10.1038/s44318-025-00559-1. Online ahead of print.

Authors

Affiliations

¹ Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, 94158, USA. Homa.Majd@ucsf.edu.
² Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, 94143, USA. Homa.Majd@ucsf.edu.
³ Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, 94158, USA.
⁴ Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, 94143, USA.
⁵ Department of Pathology, University of California, San Francisco, San Francisco, CA, USA.
⁶ Department of Pediatric Surgery, Massachusetts General Hospital, Boston, MA, USA.
⁷ Department of Anatomy and Physiology, the University of Melbourne, Parkville, VIC, Australia.
⁸ Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, 94158, USA. Faranak.Fattahi@ucsf.edu.
⁹ Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, 94143, USA. Faranak.Fattahi@ucsf.edu.
¹⁰ Program in Craniofacial Biology, University of California, San Francisco, CA, USA. Faranak.Fattahi@ucsf.edu.

^# Contributed equally.

PMID: 40954253
DOI: 10.1038/s44318-025-00559-1

No abstract available

Keywords: Comparative Analysis; Neurochemical Coding; Neuronal Classification.

PubMed Disclaimer

Conflict of interest statement

Disclosure and competing interests statement. F.F. is the inventor of several patent applications owned by UCSF, MSKCC, and Weill Cornell Medicine related to hPSC-differentiation technologies, including technologies for the derivation of enteric neurons and their application for drug discovery.

Update of

A call for a unified and multimodal definition of cellular identity in the enteric nervous system.
Majd H, Cesiulis A, Samuel RM, Richter MN, Elder N, Guyer RA, Hao MM, Stamp LA, Goldstein AM, Fattahi F. Majd H, et al. bioRxiv [Preprint]. 2024 Feb 5:2024.01.15.575794. doi: 10.1101/2024.01.15.575794. bioRxiv. 2024. Update in: EMBO J. 2025 Sep 15. doi: 10.1038/s44318-025-00559-1. PMID: 38293133 Free PMC article. Updated. Preprint.

References

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1. Argelaguet R, Cuomo ASE, Stegle O, Marioni JC (2021) Computational principles and challenges in single-cell data integration. Nat Biotechnol 39:1202–1215 - DOI - PubMed
1. Balasuriya GK, Nugapitiya SS, Hill-Yardin EL, Bornstein JC (2021) Nitric oxide regulates Estrus cycle dependent colonic motility in mice. Front Neurosci 15:647555 - DOI - PubMed - PMC
1. Barber K, Studer L, Fattahi F (2019) Derivation of enteric neuron lineages from human pluripotent stem cells. Nat Protoc 14:1261–1279 - DOI - PubMed - PMC
1. Beach DL, Keene JD (2008) Ribotrap: targeted purification of RNA-specific RNPs from cell lysates through immunoaffinity precipitation to identify regulatory proteins and RNAs. Methods Mol Biol Clifton NJ 419:69–91 - DOI

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A call for a unified and multimodal definition of cellular identity in the enteric nervous system

Affiliations

A call for a unified and multimodal definition of cellular identity in the enteric nervous system

Authors

Affiliations

Conflict of interest statement

Update of

References

Grants and funding

LinkOut - more resources

Full Text Sources

Miscellaneous