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. 2025 Oct;60(10):e5173.
doi: 10.1002/jms.5173.

Evaluation of the Chemical Profile by Paper Spray Mass Spectrometry of Eugenia uniflora Pulp, Peel, Seeds, and Jelly

Viviane D M Silva  1   2 , Laiza A Nogueira  1 , Ana Luiza C C Ramos  2 , Bruna V Nunes  2   3 , Claudio A Lopes  1 , Mauro R Silva  4 , Eric M Garcia  1 , Hosane Aparecida Taroco  1 , Ricardo Manuel S de Boavida Ferreira  3 , Rodinei Augusti  5 , Julio Onesio-Ferreira Melo  1   3
Affiliations

Evaluation of the Chemical Profile by Paper Spray Mass Spectrometry of Eugenia uniflora Pulp, Peel, Seeds, and Jelly

Viviane D M Silva et al. J Mass Spectrom. 2025 Oct.

Abstract

Pitangueira (Eugenia uniflora) is a fruit tree found in the Cerrado, Caatinga, and Atlantic Forest Brazilian biomes. Due to its intense and characteristic aroma and flavor, its fruits (Brazilian cherry) can be consumed raw and as an ingredient in jellies, giving it better use and a more excellent shelf life. The objective of this work was to evaluate the chemical profile of chemical compounds in the pulp, peel, seed, and jelly prepared with the pulp and peel of Brazilian cherry. The characterization of the chemical profile was performed by a fast and simple method: paper spray ionization method coupled with mass spectrometry in positive and negative ionization modes. Forty-six compounds were tentatively identified in the negative mode and 15 in the positive mode, belonging to the classes of phenolic acids, organic acids, fatty acids, hydroxycinnamic acids, sugars, betalains, carotenoids, flavonoids, and tannins. Characterizing these native Brazilian fruits and their products may increase interest in their use, highlighting the potential of Brazilian cherry as a source of nutrients and bioactive compounds. Therefore, developing technological processes for better use and conservation of fruits and, at the same time, identifying the phytochemical compounds present in the product is a way of valuing and preserving our Brazilian biodiversity.

Keywords: Brazilian cherry; conservation; fingerprints; phytochemicals.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

FIGURE 1
FIGURE 1
Representation of PS(+)‐MS of a sample of pulp and peel (A), seed (B), and jelly (C) of Brazilian cherry.
FIGURE 2
FIGURE 2
Representation of E. uniflora extracts in the first and second dimensions through principal component analysis (PCA) (A) and of the main ions responsible for sample separation in PC 1 (B) and PC 2 (C) in positive mode.
FIGURE 3
FIGURE 3
Representation of PS(−)‐MS of a sample of pulp and peel (A), seed (B), and jelly (C) of Brazilian cherry.
FIGURE 4
FIGURE 4
Representation of E. uniflora extracts in the first and second dimensions through principal component analysis (PCA) (A) and of the main ions responsible for sample separation in PC 1 (B) and PC 2 (C) in negative mode.
FIGURE 5
FIGURE 5
Dendrogram obtained from the analysis of hierarchical clusters (HCA) in the representation of Eugenia uniflora samples in positive mode (A) and negative mode (B).
See this image and copyright information in PMC

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