Gut microbiome comparability between DNA extraction kits

Gregory R Young¹, Nurulamin Noor^{2

3}, Aryan Khirwadkar⁴, Lauren C Beck⁴, Mohmmed Tauseef Sharip², Kevin Whelan⁵, Nicholas A Kennedy^{6

7}, Jack Satsangi^{8

9}, Julian Marchesi¹⁰, Luke Jostins-Dean¹¹, Christopher Stewart⁴, Paul A Lyons^{3

12}, Miles Parkes^{2

3}, Christopher Andrew Lamb^{13

14}; IBD-RESPONSE collaborators

Collaborators, Affiliations

Collaborators

IBD-RESPONSE collaborators:
Badr Abdalla, Natalie Agius, Tariq Ahmad, Debbie Alexander, Emma Ali, Dean Allerton, Carl Anderson, Smantha Baillie, Georgina Bates, Ian Beales, Lauren Beck, Zareen Bheekhun, Jane Biala, Jonathan Blackwell, Joanne Bone, Simon Borg-Bartolo, Louise Bowlas, Biljana Brezina, Johanne Brooks-Warburton, Amy Buckley, Helen Burton, Jeffrey Butterworth, Beatriz Camesella Perez, Jade Chisholm, Lee Choong, Bessie Cipriano, Tonia Clark, Sonya Collins, Rachel Cooney, Jessica Cordle, Cristina Cotobal Martin, J R Cummings, Ayokunnumi Dada, Helen Dallal, Kate Datta, Jiezel De Guzman, Juan de la Revilla Negro, Aminda De Silva, Anjan Dhar, Raymoncler Diaz, Benjamin Disney, Chaonan Dong, Mary Doona, Jennifer Doyle, Dharmaraj Durai, Jill Effard, Alexander Elford, Laura Fachal, Francesco Ferraro, Mary Flowerdew, Stephen Foley, Katherine Frith, Natalia Fumis, Hannah Gibson, Zaireen Mae Gico Pios, Rachel Godfrey, Kay Goulden, Neethu Gouri Das, Marie Green, Paul Harrow, Ailsa Hart, Philip Harvey, Victoria Hildreth, Hannah Husband, Peter Irving, Katherine Jacques, Lauren John, Heather Johnson, Marie Jones, Luke Jostins-Dean, Sunita Juliao, Nicholas Kennedy, Alexandra Kent, Uzma Khan, Andrew King, Klaartje Kok, Konrad Koss, Yasmin Laidler, Christopher Lamb, Isabel Laszczak, Sarah Lawrence, Emma Lee, James Lee, Irish Lee, Charlie Lees, Robert Lees, Trevor Liddle, Jimmy Limdi, James Lindsay, Dennies Lopez, Melanie Love, Mary Lucas, Hannah Mabb, Sadek Malas, Julian Marchesi, Naomi McGregor, Simon McLaughlin, Claire McQuillian, Helen Millage, Ibinye Minimah, Matthew Morris, Alison Moss, Robert Mulligan, Elaine O'Reilly, Felicia Onoviran, Debra Owens, Christopher Palmer-Jones, Simon Panter, Ji Park, Miles Parkes, Gareth Parkes, Alina Pascau, Chirag Patel, Kamal Patel, Sally Payton, Mirela Petrova, Jahleesha Pinney, Kathryn Potts, Nick Powell, Sam Powles, Kiran Prabhu, Natalie Prescott, Aisling Quinn, Eduardo Quintero, Ajaysuraj Rachuri, Chandni Radia, Monira Rahman, Sohail Rahmany, Tim Raine, Praveen Rajasekhar, Arvind Ramadas, Krithivasan Raman, Lucia Ramirez Navarro, Parizade Raymode, Jess Record, Madeleine Richardson, Susan Ritchie, Francesca Rosa, Jack Satsangi, John Saunders, Lydia Scarlett, Shaji Sebastian, Jahnavi Seema, Christian Selinger, Krishna Shah, Anita Sharda, Mohammed Sharip, Natalie Shields, Pamela Simmons, Oliver Skipper, Melissa Smith, Ally Speight, Alan Steel, Alison Stephens, Christopher Stewart, Michelle Strickland, Sreedhar Subramanian, Alison Talbot, John Thomas, Valentina Toska, Artemis Trikola, Robert Varley, Ajay Verma, Busiswa Vuyelele Titchmarsh, Hannah Watson, Andrea Webster, Emma Wesley, Kevin Whelan, Simone Whiteoak, Kate Wistance, Ruth Wood, Nicola Wyatt, Yifan Xu, Mark Yongco, Gregory Young, Louise-Jane Young

Affiliations

¹ Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, UK greg.young@newcastle.ac.uk.
² Department of Gastroenterology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK.
³ Department of Medicine, University of Cambridge, Cambridge, UK.
⁴ Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, UK.
⁵ Nutritional Sciences, King's College London, London, UK.
⁶ Gastroenterology, Royal Devon and Exeter NHS Foundation Trust, Exeter, UK.
⁷ University of Exeter, Exeter, UK.
⁸ Nuffield Department of Medicine, University of Oxford, Oxford, UK.
⁹ Centre for Genomic and Experimental Medicine, University of Edinburgh, Edinburgh, UK.
¹⁰ Division of Digestive Diseases, Department of Metabolism, Imperial College London, London, UK.
¹¹ Kennedy Institute of Rheumatology, Oxford University, Oxford, UK.
¹² Cambridge Institute for Therapeutic Immunology and Infectious Disease, Jeffrey Cheah Biomedical Centre, Cambridge, UK.
¹³ Translational and Clinical Research Institute, Newcastle University Faculty of Medical Sciences, Newcastle upon Tyne, UK.
¹⁴ Department of Gastroenterology, Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK.

PMID: 40957661
DOI: 10.1136/gutjnl-2025-336648

Gut microbiome comparability between DNA extraction kits

Gregory R Young et al. Gut. 2025.

. 2025 Sep 16:gutjnl-2025-336648.

doi: 10.1136/gutjnl-2025-336648. Online ahead of print.

Authors

Collaborators

IBD-RESPONSE collaborators:
Badr Abdalla, Natalie Agius, Tariq Ahmad, Debbie Alexander, Emma Ali, Dean Allerton, Carl Anderson, Smantha Baillie, Georgina Bates, Ian Beales, Lauren Beck, Zareen Bheekhun, Jane Biala, Jonathan Blackwell, Joanne Bone, Simon Borg-Bartolo, Louise Bowlas, Biljana Brezina, Johanne Brooks-Warburton, Amy Buckley, Helen Burton, Jeffrey Butterworth, Beatriz Camesella Perez, Jade Chisholm, Lee Choong, Bessie Cipriano, Tonia Clark, Sonya Collins, Rachel Cooney, Jessica Cordle, Cristina Cotobal Martin, J R Cummings, Ayokunnumi Dada, Helen Dallal, Kate Datta, Jiezel De Guzman, Juan de la Revilla Negro, Aminda De Silva, Anjan Dhar, Raymoncler Diaz, Benjamin Disney, Chaonan Dong, Mary Doona, Jennifer Doyle, Dharmaraj Durai, Jill Effard, Alexander Elford, Laura Fachal, Francesco Ferraro, Mary Flowerdew, Stephen Foley, Katherine Frith, Natalia Fumis, Hannah Gibson, Zaireen Mae Gico Pios, Rachel Godfrey, Kay Goulden, Neethu Gouri Das, Marie Green, Paul Harrow, Ailsa Hart, Philip Harvey, Victoria Hildreth, Hannah Husband, Peter Irving, Katherine Jacques, Lauren John, Heather Johnson, Marie Jones, Luke Jostins-Dean, Sunita Juliao, Nicholas Kennedy, Alexandra Kent, Uzma Khan, Andrew King, Klaartje Kok, Konrad Koss, Yasmin Laidler, Christopher Lamb, Isabel Laszczak, Sarah Lawrence, Emma Lee, James Lee, Irish Lee, Charlie Lees, Robert Lees, Trevor Liddle, Jimmy Limdi, James Lindsay, Dennies Lopez, Melanie Love, Mary Lucas, Hannah Mabb, Sadek Malas, Julian Marchesi, Naomi McGregor, Simon McLaughlin, Claire McQuillian, Helen Millage, Ibinye Minimah, Matthew Morris, Alison Moss, Robert Mulligan, Elaine O'Reilly, Felicia Onoviran, Debra Owens, Christopher Palmer-Jones, Simon Panter, Ji Park, Miles Parkes, Gareth Parkes, Alina Pascau, Chirag Patel, Kamal Patel, Sally Payton, Mirela Petrova, Jahleesha Pinney, Kathryn Potts, Nick Powell, Sam Powles, Kiran Prabhu, Natalie Prescott, Aisling Quinn, Eduardo Quintero, Ajaysuraj Rachuri, Chandni Radia, Monira Rahman, Sohail Rahmany, Tim Raine, Praveen Rajasekhar, Arvind Ramadas, Krithivasan Raman, Lucia Ramirez Navarro, Parizade Raymode, Jess Record, Madeleine Richardson, Susan Ritchie, Francesca Rosa, Jack Satsangi, John Saunders, Lydia Scarlett, Shaji Sebastian, Jahnavi Seema, Christian Selinger, Krishna Shah, Anita Sharda, Mohammed Sharip, Natalie Shields, Pamela Simmons, Oliver Skipper, Melissa Smith, Ally Speight, Alan Steel, Alison Stephens, Christopher Stewart, Michelle Strickland, Sreedhar Subramanian, Alison Talbot, John Thomas, Valentina Toska, Artemis Trikola, Robert Varley, Ajay Verma, Busiswa Vuyelele Titchmarsh, Hannah Watson, Andrea Webster, Emma Wesley, Kevin Whelan, Simone Whiteoak, Kate Wistance, Ruth Wood, Nicola Wyatt, Yifan Xu, Mark Yongco, Gregory Young, Louise-Jane Young

Affiliations

¹ Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, UK greg.young@newcastle.ac.uk.
² Department of Gastroenterology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK.
³ Department of Medicine, University of Cambridge, Cambridge, UK.
⁴ Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, UK.
⁵ Nutritional Sciences, King's College London, London, UK.
⁶ Gastroenterology, Royal Devon and Exeter NHS Foundation Trust, Exeter, UK.
⁷ University of Exeter, Exeter, UK.
⁸ Nuffield Department of Medicine, University of Oxford, Oxford, UK.
⁹ Centre for Genomic and Experimental Medicine, University of Edinburgh, Edinburgh, UK.
¹⁰ Division of Digestive Diseases, Department of Metabolism, Imperial College London, London, UK.
¹¹ Kennedy Institute of Rheumatology, Oxford University, Oxford, UK.
¹² Cambridge Institute for Therapeutic Immunology and Infectious Disease, Jeffrey Cheah Biomedical Centre, Cambridge, UK.
¹³ Translational and Clinical Research Institute, Newcastle University Faculty of Medical Sciences, Newcastle upon Tyne, UK.
¹⁴ Department of Gastroenterology, Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK.

PMID: 40957661
DOI: 10.1136/gutjnl-2025-336648

No abstract available

Keywords: CROHN'S DISEASE; GASTROINTESTINAL MICROBIOME; INFLAMMATORY BOWEL DISEASE; MICROBIOME; ULCERATIVE COLITIS.

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Conflict of interest statement

Competing interests: None declared.

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Gut microbiome comparability between DNA extraction kits

Collaborators

Affiliations

Gut microbiome comparability between DNA extraction kits

Authors

Collaborators

Affiliations

Conflict of interest statement

Publication types

LinkOut - more resources

Full Text Sources