Prognostic Value of Multicolor-Flow Cytometric Measurable Residual Disease Detection in Acute Myeloid Leukemia Prior to Allogeneic Hematopoietic Cell Transplantation: Potential Role of HLA-DR

Maximilian A Röhnert¹, Chiara Löffler², Michael Kramer³, Christoph Röllig⁴, Johannes Schetelig⁵, Friedrich Stölzel⁶, Desirée Kunadt⁴, Jan Moritz Middeke⁴, Lars Thielecke⁷, Friedemann Uschner⁷, Ingmar Glauche⁷, Christian Thiede⁸, Martin Bornhäuser⁹, Uta Oelschlägel⁴, Malte von Bonin⁴, Jonas Schadt⁴

Affiliations

¹ Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany. Electronic address: Maximilian-Alexander.Roehnert@uniklinikum-dresden.de.
² Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany; Else Kroener Fresenius Center for Digital Health, TUD Dresden University of Technology, Dresden, Germany; National Center for Tumor Diseases Dresden (NCT/UCC), a partnership between DKFZ, Carl Gustav Carus Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.
³ AvenCell Europe GmbH, Dresden, Germany.
⁴ Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany.
⁵ Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany; DKMS Clinical Trials Unit, Dresden, Germany.
⁶ Carl Gustav Carus Faculty of Medicine, TUD Dresden University of Technology, Dresden, Germany; Division of Stem Cell Transplantation and Cellular Immunotherapies, Department of Medicine II, University Hospital Schleswig-Holstein, Kiel, Germany.
⁷ Institute for Medical Informatics and Biometry (IMB), Carl Gustav Carus Faculty of Medicine, TUD Dresden University of Technology, Dresden, Germany.
⁸ Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany; AgenDix GmbH, Dresden, Germany.
⁹ Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany; National Center for Tumor Diseases Dresden (NCT/UCC), a partnership between DKFZ, Carl Gustav Carus Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.

PMID: 40957749
DOI: 10.1016/j.clml.2025.08.013

Free article

Prognostic Value of Multicolor-Flow Cytometric Measurable Residual Disease Detection in Acute Myeloid Leukemia Prior to Allogeneic Hematopoietic Cell Transplantation: Potential Role of HLA-DR

Maximilian A Röhnert et al. Clin Lymphoma Myeloma Leuk. 2025 Dec.

Free article

. 2025 Dec;25(12):e1108-e1119.e2.

doi: 10.1016/j.clml.2025.08.013. Epub 2025 Aug 20.

Authors

Affiliations

¹ Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany. Electronic address: Maximilian-Alexander.Roehnert@uniklinikum-dresden.de.
² Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany; Else Kroener Fresenius Center for Digital Health, TUD Dresden University of Technology, Dresden, Germany; National Center for Tumor Diseases Dresden (NCT/UCC), a partnership between DKFZ, Carl Gustav Carus Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.
³ AvenCell Europe GmbH, Dresden, Germany.
⁴ Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany.
⁵ Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany; DKMS Clinical Trials Unit, Dresden, Germany.
⁶ Carl Gustav Carus Faculty of Medicine, TUD Dresden University of Technology, Dresden, Germany; Division of Stem Cell Transplantation and Cellular Immunotherapies, Department of Medicine II, University Hospital Schleswig-Holstein, Kiel, Germany.
⁷ Institute for Medical Informatics and Biometry (IMB), Carl Gustav Carus Faculty of Medicine, TUD Dresden University of Technology, Dresden, Germany.
⁸ Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany; AgenDix GmbH, Dresden, Germany.
⁹ Department of Medicine I, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany; National Center for Tumor Diseases Dresden (NCT/UCC), a partnership between DKFZ, Carl Gustav Carus Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.

PMID: 40957749
DOI: 10.1016/j.clml.2025.08.013

Abstract

We implemented a standardized multicolor-flow cytometry approach to monitor MRD in AML patients undergoing alloHCT. MRD positivity predicted inferior OS and RFS, surpassing cytomorphology. Notably, HLA-DR expression on residual leukemic cells was associated with favorable outcomes, highlighting the role of immunogenicity. Comprehensive MRD assessment may enable personalized post-transplant strategies.

Keywords: AML; HLA-DR; Immunogenicity; MFC; MRD; Risk stratification; alloHCT.

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Disclosure All authors have nothing to disclose.

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Prognostic Value of Multicolor-Flow Cytometric Measurable Residual Disease Detection in Acute Myeloid Leukemia Prior to Allogeneic Hematopoietic Cell Transplantation: Potential Role of HLA-DR

Affiliations

Prognostic Value of Multicolor-Flow Cytometric Measurable Residual Disease Detection in Acute Myeloid Leukemia Prior to Allogeneic Hematopoietic Cell Transplantation: Potential Role of HLA-DR

Authors

Affiliations

Abstract

Conflict of interest statement

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Medical

Research Materials