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. 1970 Oct 30;170(3957):550-1.
doi: 10.1126/science.170.3957.550.

Antibody to nuclear material eluted from isolated spleen vessels in systemic lupus erythematosus

K H Svec, S T Allen

Antibody to nuclear material eluted from isolated spleen vessels in systemic lupus erythematosus

K H Svec et al. Science. .

Abstract

Splenic blood vessels were isolated from surrounding cells by treatment with trypsin and the use of ultrasound. This procedure retained vascular-bound immunoglobulins which were recovered by acid elution of vessels isolated from a patient with systemic lupus erythematosus. These eluted immunoglobulins reacted with material from cell nuclei as demonstrated by immunofluorescence.

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