Effect of root conditioning agents on teeth periodontal using Scanning electron microscopy

Abstract

Mechanical instrumentation of root surfaces results in the formation of a smear layer that may impede the reattachment of connective tissue. Root conditioning agents are employed to remove the smear layer, expose collagen fibres and facilitate improved tissue regeneration. Therefore, it is of interest to assess the efficacy of five root conditioning agents-20% citric acid, 17% EDTA, 37% phosphoric acid, 250 mg/mL tetracycline hydrochloride and 0.8% hyaluronic acid-using scanning electron microscopy. Tetracycline hydrochloride showed the greatest efficacy, exhibiting a significantly higher number of patent dentinal tubules compared to other agents. Thus, root surface conditioning enhances biocompatibility, promotes fibroblast adhesion and contributes to favourable periodontal healing and successful surgical outcomes.

Keywords: EDTA; Hyaluronic acid; Phosphoric acid; Root Conditioning; Scanning Electron Microscopy (SEM); Tetracycline HCL; citric acid; periodontal disease.