Electron microscopic observations in subacute sclerosing panencephalitis brain cell cultures: their correlation with cytochemical and immunocytological findings

Abstract

Three cell cultures established from brain tissue obtained by biopsy of patients with subacute sclerosing panencephalitis (SSPE) were studied with the electron microscope in an attempt to correlate ultrastructural changes with those found by cytochemistry and immunocytology. These cells contained a large number of nucleocapsids resembling those of a paramyxovirus concentrated in the nuclear inclusions, but also seen free in the nucleus and occasionally in the cytoplasmic inclusions. Nuclear bodies associated with the nucleocapsids and granular filaments occupied a vast area of the cytoplasm. The nuclear inclusions containing nucleocapsids corresponded to the eosinophilic and fluorescent nuclear inclusions. The areas occupied by granular filaments corresponded to the diffuse cytoplasmic fluorescence. The ultrastructural changes were similar to those seen in the original brain biopsies. In addition papova-like virions were noted in brain cell cultures derived from a biopsy but not in the brain tissue itself. Their relationship to SSPE remains undetermined.