Order of the two major head protein genes of bacteriophage phi 29 of Bacillus subtilis
- PMID: 409855
- PMCID: PMC515938
- DOI: 10.1128/JVI.24.1.378-382.1977
Order of the two major head protein genes of bacteriophage phi 29 of Bacillus subtilis
Abstract
Bacteriophage phi 29 mutation sus8(22) has been mapped by two-factor crosses between markers sus8(769) and ts8(93). Whe sus8(22) infects Bacillus subtilis su- proteins, HP1 (major head protein) and HP3 (fiber protein) are not synthesized; instead, a fragment with a molecular weight of 25,000 is produced. The tryptic peptides of the fragments overlap with corresponding peptides in protein HP1, but not with the peptides of protein HP3, showing that cistron 8 codes for the major head protein HP1.
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