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. 1970 Jul;19(1):1-10.

Studies on nuclear staining by the fluorescent antibody method. I. Non-immunological factors leading to nuclear fluorescence

D Bruchhausen, R Hofermann, H von Mayersbach

Studies on nuclear staining by the fluorescent antibody method. I. Non-immunological factors leading to nuclear fluorescence

D Bruchhausen et al. Immunology. 1970 Jul.

Abstract

The causes of non-specific nuclear staining in the fluorescent antibody method were investigated. Minute amounts of labelled basic proteins, present in labelled sera, stain nuclei in both direct and indirect methods.

The attraction of these basic proteins is caused by electrostatical forces, binding basic items to the nuclei like basic dyes.

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