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. 1971 Jan 8;171(3966):75-7.
doi: 10.1126/science.171.3966.75.

Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma

B Mohit, K Fan

Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma

B Mohit et al. Science. .

Abstract

A hybrid cell line was established by cell fusion between a cloned Balbic myeloma that is resistant to 8-azaguanine and produces immunoglobulin (gammaG and free kappa chain) and C57BL/6N lymphoma that is resistant to bromo-deoxyuridine and does not produce immunoglobulins. The hybrid cells contained the membrane antigens of both parents; they synthesized free kappa chain; no synthesis of gammaG (gamma(2a)) heavy chain was detected.

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