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. 2025 Jul;179(3):363-367.
doi: 10.1007/s10517-025-06490-z. Epub 2025 Sep 25.

Hematological Parameters in Colitis-Associated Colorectal Cancer in Animals with Different Resistance to Hypoxia

D Sh Dzhalilova  1 , M V Silina  2 , I S Tsvetkov  2 , O V Makarova  2
Affiliations

Hematological Parameters in Colitis-Associated Colorectal Cancer in Animals with Different Resistance to Hypoxia

D Sh Dzhalilova et al. Bull Exp Biol Med. 2025 Jul.

Abstract

Hematological parameters were evaluated, proinflammatory immune response indices lymphocyte-to-monocyte ratio (LMR) and neutrophil-to-lymphocyte ratio (NLR) were calculated, and iron was detected in tumor tissues by Perls' staining of histological sections in high (HR) and low resistant (LR) to hypoxia male C57BL/6 mice with colitis-associated colorectal cancer (CAC) induced by intraperitoneal injection of azoxymethane and three cycles of consumption of dextran sulfate sodium. The animals were euthanized on day 141 after azoxymethane administration. The relative number of monocytes in the blood increased in HR mice of the experimental group in comparison with the control group, and was higher than in LR animals. In LR mice with CAC, the relative number of lymphocytes and monocytes, absolute number of monocytes in the blood decreased and the NLR increased in comparison with the control; the LMR was higher than in HR animals. Regardless of hypoxia resistance, the mice developed normocytic hypochromic anemia; iron accumulation in the tumor was observed only in LR animals. The obtained data on more pronounced changes in hematological parameters in LR animals should be taken into account in the development of new approaches to the inflammatory and tumor diseases therapy, including CAC.

Keywords: anemia; colitis-associated colorectal cancer; inflammatory response; iron; resistance to hypoxia.

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References

    1. Schmitt M, Greten FR. The inflammatory pathogenesis of colorectal cancer. Nat Rev Immunol. 2021;21(10):653-67. https://doi.org/10.1038/s41577-021-00534-x - DOI - PubMed
    1. Korbecki J, Simińska D, Gąssowska-Dobrowolska M, et al. Chronic and cycling hypoxia: drivers of cancer chronic inflammation through HIF-1 and NF-κB activation: a review of the molecular mechanisms. Int J Mol Sci. 2021;22(19):10701. https://doi.org/10.3390/ijms221910701 - DOI - PubMed - PMC
    1. Dzhalilova D, Kosyreva A, Vishnyakova P, et al. Age-related differences in hypoxia-associated genes and cytokine profile in male Wistar rats. Heliyon. 2021;7(9):e08085. https://doi.org/10.1016/j.heliyon.2021.e08085 - DOI - PubMed - PMC
    1. Dzhalilova D, Silina M, Tsvetkov I, et al. Changes in the expression of genes regulating the response to hypoxia, inflammation, cell cycle, apoptosis, and epithelial barrier functioning during colitis-associated colorectal cancer depend on individual hypoxia tolerance. Int J Mol Sci. 2024;25(14):7801. https://doi.org/10.3390/ijms25147801 - DOI - PubMed - PMC
    1. Murata M. Inflammation and cancer. Environ Health Prev Med. 2018;23(1):50. https://doi.org/10.1186/s12199-018-0740-1 - DOI - PubMed - PMC

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