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. 2025 Sep 25:e70120.
doi: 10.1002/kjm2.70120. Online ahead of print.

Involvement of the miR-128-3p/KDM3A/NLRP3 Axis in High Glucose-Induced Inflammatory Injury in Retinal Endothelial Cells

Wei-Ming Wen  1 , Jian-Bo Feng  2 , Yin-Sheng Cai  2 , Nan Lin  2 , Fei Lv  2 , Zhu-Sheng Guo  1
Affiliations
Free article

Involvement of the miR-128-3p/KDM3A/NLRP3 Axis in High Glucose-Induced Inflammatory Injury in Retinal Endothelial Cells

Wei-Ming Wen et al. Kaohsiung J Med Sci. .
Free article

Abstract

This study explores the regulatory mechanism of the miR-128-3p in diabetic retinopathy (DR)-associated inflammatory injury. A cellular model of DR was established by inducing immortalized human retinal endothelial cells (IM-HRECs) with high-glucose (HG). Cell viability was evaluated by CCK-8 assay, and the levels of TNF-α, IL-1β, and IL-10 were measured by ELISA. RT-qPCR was performed to determine miR-128-3p expression, and miR-128-3p mimics were transfected into cells to verify its regulatory role in DR-associated inflammatory injury. miR-128-3p was predicted by Starbase to bind to the 3' UTR of KDM3A, which was verified by dual-luciferase assay. The expressions of KDM3A and NLRP3 in cells were examined by Western blotting, and the enrichment of KDM3A and H3K9me2 on the NLRP3 promoter was measured by Ch-IP assay. The results revealed that HG treatment significantly reduced both IM-HREC viability and IL-10 levels, increased the levels of TNF-α and IL-1β, and downregulated the expression of miR-128-3p. Overexpression of miR-128-3p reduced inflammation in IM-HRECs induced by HG. The proposed mechanism involves targeting of the KDM3A 3' UTR by miR-128-3p, leading to reduced KDM3A expression, while KDM3A increased NLRP3 expression by reducing H3K9me2. In conclusion, upregulation of miR-128-3p increases the histone H3K9me2 level by inhibiting KDM3A expression, thereby reducing NLRP3 expression and suppressing DR inflammatory injury.

Keywords: H3K9me2; KDM3A; diabetic retinopathy; inflammatory injury; miR‐128‐3p.

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