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. 1977 Jul;4(7):2283-92.
doi: 10.1093/nar/4.7.2283.

A rapid cytosine-specific modification of E. coli tRNA Leu 1 by semicarbazide-bisulfite, a probe for polynucleotide conformations

K Negishi, F Harada, S Nishimura, H Hayatsu
Free PMC article

A rapid cytosine-specific modification of E. coli tRNA Leu 1 by semicarbazide-bisulfite, a probe for polynucleotide conformations

K Negishi et al. Nucleic Acids Res. 1977 Jul.
Free PMC article

Abstract

Cytosine residues in 32P-labeled E. coli tRNA Leu 1 were modified by treatment of the tRNA with the semicarbazide-bisulfite reagents [Hayatsu, H. (1976) Biochemistry 15, 2677-2682]. Analysis of the modification sites showed that only four cytidine residues, i.e. C35, C53, C85 and C86, reacted. They were identical with the cytidines of this tRNA accessible to methoxyamine [Chang, S. E. and Ish-Horowicz, D. (1974) J. Mol. Biol. 84, 375-388] and the accessibility was consistent with the conformational features recognized for tRNA in general. The rapidity and the simple nature of this modification demonstrate that the semicarbazide-bisulfite reaction is a useful tool in studying conformations of polynucleotides.

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References

    1. J Biol Chem. 1972 Jun 25;247(12):3864-7 - PubMed
    1. Nucleic Acids Res. 1976 Dec;3(12):3383-96 - PubMed
    1. J Biol Chem. 1973 Aug 10;248(15):5549-51 - PubMed
    1. J Mol Biol. 1971 Jul 28;59(2):359-73 - PubMed
    1. J Mol Biol. 1973 Apr 15;75(3):533-47 - PubMed